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A sperm suspension from cauda epididymides of Sprague-Dawley (SD) rats was prepared with or without ultrasonic treatment. The sonicated and non-sonicated sperm suspensions were processed for freeze-thawing (FT groups; 100 mu l sample was cooled in liquid nitrogen vapor, stored for 1 day at 196 degrees C, and thawed in a 25 degrees C water bath) and freeze-drying (FD groups; 100 mu l sample was frozen in liquid nitrogen for 20s, lyophilized for 6 h, stored at 4 degrees C for 2 days, and rehydrated with 100 mu l ultrapure water), or were subjected to immediate use for ICSI (fresh control groups). The sperm heads were microinjected into denuded SD oocytes using a piezo-driven micropipette 2-4 mu m in diameter. The presumptive zygotes were transferred into oviducts of pseudopregnant Wistar female rats. Viable rat offspring were produced from all six experimental groups. Ultrasonic treatment of rat spermatozoa. was effective in increasing the offspring rate (23.3% vs 6.7% in fresh control groups, 35.0% vs 7.6% in FT groups, 9.2% vs 2.5% in FD groups). The acrosomal region appeared to be intact even after ultrasonic FT and FD treatments as well as in the fresh controls, while the lateral dorsal region of the sperm membrane was more or less damaged in the sonicated, FT and FD samples. Thus, the successful participation of freeze-dried spermatozoa in full-term development was demonstrated by applying ICSI in the rat."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hirabayashi, M"}], "nameIdentifiers": [{"nameIdentifier": "41499", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kato, M"}], "nameIdentifiers": [{"nameIdentifier": "41500", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ito, J"}], "nameIdentifiers": [{"nameIdentifier": "41501", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hochi, S"}], "nameIdentifiers": [{"nameIdentifier": "41502", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ZYG13(1)79-85(\u002705).pdf", "filesize": [{"value": "96.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 96200.0, "url": {"label": "ZYG13(1)79-85(\u002705).pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/13558/files/ZYG13(1)79-85(\u002705).pdf"}, "version_id": "3aa282fd-eed1-47cf-8ef1-0cb9bab55e9b"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Viable rat offspring derived from oocytes intracytoplasmically injected with freeze-dried sperm heads", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Viable rat offspring derived from oocytes intracytoplasmically injected with freeze-dried sperm heads", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1310"], "permalink_uri": "http://hdl.handle.net/10091/270", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2007-08-28"}, "publish_date": "2007-08-28", "publish_status": "0", "recid": "13558", "relation": {}, "relation_version_is_last": true, "title": ["Viable rat offspring derived from oocytes intracytoplasmically injected with freeze-dried sperm heads"], "weko_shared_id": -1}
Viable rat offspring derived from oocytes intracytoplasmically injected with freeze-dried sperm heads
http://hdl.handle.net/10091/270
http://hdl.handle.net/10091/270cf375755-698b-4e3a-be89-c3dd1680d7e6
名前 / ファイル | ライセンス | アクション |
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ZYG13(1)79-85('05).pdf (96.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-08-28 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Viable rat offspring derived from oocytes intracytoplasmically injected with freeze-dried sperm heads | |||||
言語 | ||||||
言語 | eng | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1017/S096719940500300X | |||||
関連名称 | 10.1017/S096719940500300X | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Hirabayashi, M
× Hirabayashi, M× Kato, M× Ito, J× Hochi, S |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Hochi, S | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html | |||||
出版者 | ||||||
出版者 | CAMBRIDGE UNIV PRESS | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | ZYGOTE. 13(1): 79-85 (2005) | |||||
書誌情報 |
Zygote 巻 13, 号 1, p. 79-85, 発行日 2005-02 |
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資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0967-1994 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10958262 | |||||
他の資源との関係:URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://journals.cambridge.org/action/displayJournal?jid=ZYG | |||||
関連名称 | http://journals.cambridge.org/action/displayJournal?jid=ZYG | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000229752800012 |