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This method has remained practically unchanged for more than 20 years, but the roles these molecules play are not definitively understood. To gain a better understanding, we have compared PEG transformation to a protocol using polyethylenimine (PEI) that is the basis for non-viral transfection in mammals and which has a well established molecular model for assisting DNA uptake. Protoplasts of Aspergillus nidulans could be transformed in the presence of Ca2+ with a relatively high ratio of PEI to DNA molecules. By comparing PEI and PEG in terms of interaction with DNA, fungal protoplasts, and response to different transformation conditions, we propose that the role of PEG is most likely to function after transforming DNA is incorporated into protoplasts, rather than the accepted view that it functions outside of the cell. 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Comparison between polyethylene glycol- and polyethylenimine-mediated transformation of Aspergillus nidulans
http://hdl.handle.net/10091/1153
http://hdl.handle.net/10091/1153bf6594ef-566b-47dd-b287-16a89167e11a
名前 / ファイル | ライセンス | アクション |
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Kuwanoetalrevision[1].pdf (544.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-09-11 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Comparison between polyethylene glycol- and polyethylenimine-mediated transformation of Aspergillus nidulans | |||||
言語 | ||||||
言語 | eng | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1007/s00294-008-0204-z | |||||
関連名称 | 10.1007/s00294-008-0204-z | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Kuwano, T
× Kuwano, T× Shirataki, C× Itoh, Y |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Itoh, Y | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.OVTNOUkh.html | |||||
出版者 | ||||||
出版者 | SPRINGER | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | CURRENT GENETICS. 54(2):95-103(2008) | |||||
書誌情報 |
CURRENT GENETICS 巻 54, 号 2, p. 95-103, 発行日 2008-07 |
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資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0172-8083 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00146001 | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000258152800004 |