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Immunoelectron Microscopic Observation of Chicken Glucagon-Like Peptide (GLP)-1-Containing Cells in Tissues Derived from Thin Section, Paraffin Block and Conventional Method
http://hdl.handle.net/10091/17856
http://hdl.handle.net/10091/178567b4ba2e0-f890-4985-a4af-42647183080e
名前 / ファイル | ライセンス | アクション |
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Immunoelectron_Microscopic_Observation_Chicken_Glucagon-Like.pdf (3.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-09-05 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Immunoelectron Microscopic Observation of Chicken Glucagon-Like Peptide (GLP)-1-Containing Cells in Tissues Derived from Thin Section, Paraffin Block and Conventional Method | |||||
言語 | ||||||
言語 | eng | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1292/jvms.13-0339 | |||||
関連名称 | 10.1292/jvms.13-0339 | |||||
キーワード | ||||||
主題 | glucagon-like peptide-1, immunocytochemistry, paraffin-embedded tissue, reprocessing method, transmission electron microscope | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Watanabe, Takafumi
× Watanabe, Takafumi× Nishimura, Kei× Monir, Mohammad M.× Takemoto, Chihiro× Hiramatsu, Kohzy |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Hiramatsu, Kohzy | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.yeTmPpkh.html | |||||
出版者 | ||||||
出版者 | JAPAN SOC VET SCI | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | JOURNAL OF VETERINARY MEDICAL SCIENCE. 76(3):389-394 (2014) | |||||
書誌情報 |
JOURNAL OF VETERINARY MEDICAL SCIENCE 巻 76, 号 3, p. 389-394, 発行日 2014-03 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The purpose of the present study was to investigate the possibility of immunoelectron microscopic observation of endocrine cells in paraffin-embedded tissues. The procedure, which involves reprocessing from sliced tissues and immunohistochemical staining by colloidal-gold immunolabeling of paraffin sections from paraffin blocks, was able to reveal the fine characteristics of secretory granules containing glucagon-like peptide-1. Morphometric analyses of the secretory granules showed no significant differences between the reprocessing procedure and a conventional post-embedding procedure, which was performed as a control. The reprocessing procedure has some advantages besides providing information on secretory granules containing the amino acid peptide. For example, the same cell can be observed under both a light microscope and the electron microscope. In addition, the high-electron densities of silver-enhanced gold particles are easily recognized, and the boundary between the profile of the granules and the immunogold labeling is clearly shown at the electron microscopic level. Furthermore, the procedure, which is inexpensive and does not require special devices, can effectively use precious samples that are already paraffin-embedded and unable to be obtained twice, such as the case for endangered animals and rare pathological tissues. To the best of our knowledge, the present study is the first to report the advantages of the reprocessing method for sliced paraffin sections of gut endocrine cells. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0916-7250 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10796138 | |||||
PubMed | ||||||
識別子タイプ | PMID | |||||
関連識別子 | https://pubmed.ncbi.nlm.nih.gov/24257328/ | |||||
関連名称 | 24257328 | |||||
権利 | ||||||
権利情報 | Copyright© 2014 The Japanese Society of Veterinary Science | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000333777400010 |