WEKO3
アイテム
{"_buckets": {"deposit": "ffae6be0-bdce-4f57-8105-2cdef04d18e9"}, "_deposit": {"id": "13378", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "13378"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00013378", "sets": ["1310"]}, "author_link": ["40639", "40640", "40641", "40642", "40643", "40644", "40645"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012-03-23", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "13", "bibliographicPageEnd": "9776", "bibliographicPageStart": "9765", "bibliographicVolumeNumber": "287", "bibliographic_titles": [{"bibliographic_title": "JOURNAL OF BIOLOGICAL CHEMISTRY"}]}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Cell wall metabolism and cell wall modification are very important processes that bacteria use to adjust to various environmental conditions. One of the main modifications is deacetylation of peptidoglycan. The polysaccharide deacetylase homologue, Bacillus subtilis YjeA (renamed PdaC), was characterized and found to be a unique deacetylase. The pdaC deletion mutant was sensitive to lysozyme treatment, indicating that PdaC acts as a deacetylase. The purified recombinant and truncated PdaC from Escherichia coli deacetylated B. subtilis peptidoglycan and its polymer, (-GlcNAc-MurNAc[-L-Ala-D-Glu]-)(n). Surprisingly, RP-HPLC and ESI-MS/MS analyses showed that the enzyme deacetylates N-acetylmuramic acid (MurNAc) not GlcNAc from the polymer. Contrary to Streptococcus pneumoniae PgdA, which shows high amino acid sequence similarity with PdaC and is a zinc-dependent GlcNAc deacetylase toward peptidoglycan, there was less dependence on zinc ion for deacetylation of peptidoglycan by PdaC than other metal ions (Mn2+, Mg2+, Ca2+). The kinetic values of the activity toward B. subtilis peptidoglycan were K-m = 4.8 mM and k(cat) = 0.32 s(-1). PdaC also deacetylated N-acetylglucosamine (GlcNAc) oligomers with a K-m = 12.3 mM and k(cat) = 0.24 s(-1) toward GlcNAc(4). Therefore, PdaC has GlcNAc deacetylase activity toward GlcNAc oligomers and MurNAc deacetylase activity toward B. subtilis peptidoglycan.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "資源タイプ(コンテンツの種類)", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "JOURNAL OF BIOLOGICAL CHEMISTRY. 287(13):9765-9776 (2012)", "subitem_description_type": "Other"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000302167200010"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "22277649"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search\u0026DB=pubmed\u0026term=22277649", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1074/jbc.M111.329490"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1074/jbc.M111.329490", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Copyright© 2012 The American Society for Biochemistry and Molecular Biology, Inc."}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0021-9258", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0021-9258", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00251083", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "N-ACETYLGLUCOSAMINE DEACETYLASE; YYCFG 2-COMPONENT SYSTEM; SENSOR HISTIDINE KINASE; CHITIN DEACETYLASE; COLLETOTRICHUM-LINDEMUTHIANUM; STREPTOCOCCUS-PNEUMONIAE; PEPTIDOGLYCAN STRUCTURE; ENTEROCOCCUS-FAECALIS; VIRULENCE FACTOR; CELL-DIVISION"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Cell wall metabolism and cell wall modification are very important processes that bacteria use to adjust to various environmental conditions. One of the main modifications is deacetylation of peptidoglycan. The polysaccharide deacetylase homologue, Bacillus subtilis YjeA (renamed PdaC), was characterized and found to be a unique deacetylase. The pdaC deletion mutant was sensitive to lysozyme treatment, indicating that PdaC acts as a deacetylase. The purified recombinant and truncated PdaC from Escherichia coli deacetylated B. subtilis peptidoglycan and its polymer, (-GlcNAc-MurNAc[-L-Ala-D-Glu]-)(n). Surprisingly, RP-HPLC and ESI-MS/MS analyses showed that the enzyme deacetylates N-acetylmuramic acid (MurNAc) not GlcNAc from the polymer. Contrary to Streptococcus pneumoniae PgdA, which shows high amino acid sequence similarity with PdaC and is a zinc-dependent GlcNAc deacetylase toward peptidoglycan, there was less dependence on zinc ion for deacetylation of peptidoglycan by PdaC than other metal ions (Mn2+, Mg2+, Ca2+). The kinetic values of the activity toward B. subtilis peptidoglycan were K-m = 4.8 mM and k(cat) = 0.32 s(-1). PdaC also deacetylated N-acetylglucosamine (GlcNAc) oligomers with a K-m = 12.3 mM and k(cat) = 0.24 s(-1) toward GlcNAc(4). Therefore, PdaC has GlcNAc deacetylase activity toward GlcNAc oligomers and MurNAc deacetylase activity toward B. subtilis peptidoglycan."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kobayashi, Kaori"}], "nameIdentifiers": [{"nameIdentifier": "40639", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sudiarta, I. Putu"}], "nameIdentifiers": [{"nameIdentifier": "40640", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kodama, Takeko"}], "nameIdentifiers": [{"nameIdentifier": "40641", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fukushima, Tatsuya"}], "nameIdentifiers": [{"nameIdentifier": "40642", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ara, Katsutoshi"}], "nameIdentifiers": [{"nameIdentifier": "40643", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ozaki, Katsuya"}], "nameIdentifiers": [{"nameIdentifier": "40644", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sekiguchi, Junichi"}], "nameIdentifiers": [{"nameIdentifier": "40645", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Identification_characterization_novel_polysaccharide_deacetylase_C.pdf", "filesize": [{"value": "475.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 475500.0, "url": {"label": "Identification_characterization_novel_polysaccharide_deacetylase_C.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/13378/files/Identification_characterization_novel_polysaccharide_deacetylase_C.pdf"}, "version_id": "9f01bc6b-e7bd-4480-95ed-fc5a399cdb7a"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Identification and Characterization of a Novel Polysaccharide Deacetylase C (PdaC) from Bacillus subtilis", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Identification and Characterization of a Novel Polysaccharide Deacetylase C (PdaC) from Bacillus subtilis", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1310"], "permalink_uri": "http://hdl.handle.net/10091/16189", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2013-01-18"}, "publish_date": "2013-01-18", "publish_status": "0", "recid": "13378", "relation": {}, "relation_version_is_last": true, "title": ["Identification and Characterization of a Novel Polysaccharide Deacetylase C (PdaC) from Bacillus subtilis"], "weko_shared_id": -1}
Identification and Characterization of a Novel Polysaccharide Deacetylase C (PdaC) from Bacillus subtilis
http://hdl.handle.net/10091/16189
http://hdl.handle.net/10091/161891f37bec9-f14c-4384-a48f-59823094b983
名前 / ファイル | ライセンス | アクション |
---|---|---|
Identification_characterization_novel_polysaccharide_deacetylase_C.pdf (475.5 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2013-01-18 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Identification and Characterization of a Novel Polysaccharide Deacetylase C (PdaC) from Bacillus subtilis | |||||
言語 | ||||||
言語 | eng | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1074/jbc.M111.329490 | |||||
関連名称 | 10.1074/jbc.M111.329490 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Kobayashi, Kaori
× Kobayashi, Kaori× Sudiarta, I. Putu× Kodama, Takeko× Fukushima, Tatsuya× Ara, Katsutoshi× Ozaki, Katsuya× Sekiguchi, Junichi |
|||||
出版者 | ||||||
出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | JOURNAL OF BIOLOGICAL CHEMISTRY. 287(13):9765-9776 (2012) | |||||
書誌情報 |
JOURNAL OF BIOLOGICAL CHEMISTRY 巻 287, 号 13, p. 9765-9776, 発行日 2012-03-23 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Cell wall metabolism and cell wall modification are very important processes that bacteria use to adjust to various environmental conditions. One of the main modifications is deacetylation of peptidoglycan. The polysaccharide deacetylase homologue, Bacillus subtilis YjeA (renamed PdaC), was characterized and found to be a unique deacetylase. The pdaC deletion mutant was sensitive to lysozyme treatment, indicating that PdaC acts as a deacetylase. The purified recombinant and truncated PdaC from Escherichia coli deacetylated B. subtilis peptidoglycan and its polymer, (-GlcNAc-MurNAc[-L-Ala-D-Glu]-)(n). Surprisingly, RP-HPLC and ESI-MS/MS analyses showed that the enzyme deacetylates N-acetylmuramic acid (MurNAc) not GlcNAc from the polymer. Contrary to Streptococcus pneumoniae PgdA, which shows high amino acid sequence similarity with PdaC and is a zinc-dependent GlcNAc deacetylase toward peptidoglycan, there was less dependence on zinc ion for deacetylation of peptidoglycan by PdaC than other metal ions (Mn2+, Mg2+, Ca2+). The kinetic values of the activity toward B. subtilis peptidoglycan were K-m = 4.8 mM and k(cat) = 0.32 s(-1). PdaC also deacetylated N-acetylglucosamine (GlcNAc) oligomers with a K-m = 12.3 mM and k(cat) = 0.24 s(-1) toward GlcNAc(4). Therefore, PdaC has GlcNAc deacetylase activity toward GlcNAc oligomers and MurNAc deacetylase activity toward B. subtilis peptidoglycan. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0021-9258 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00251083 | |||||
PubMed | ||||||
識別子タイプ | PMID | |||||
関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=22277649 | |||||
関連名称 | 22277649 | |||||
権利 | ||||||
権利情報 | Copyright© 2012 The American Society for Biochemistry and Molecular Biology, Inc. | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000302167200010 |