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Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis
http://hdl.handle.net/10091/00019348
http://hdl.handle.net/10091/00019348272432c7-24ed-434e-9571-b698edbb3fd5
名前 / ファイル | ライセンス | アクション |
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Secretion_immunoreactive_single-chain_variable_fragment.pdf (4.8 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-02-01 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Immunoreactivity, Interleukin 6, Lactococcus lactis, gmLAB, scFv antibody | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Shigemori, Suguru
× Shigemori, Suguru× Ihara, Masaki× Sato, Takashi× Yamamoto, Yoshinari× Nigar, Shireen× Ogita, Tasuku× Shimosato, Takeshi |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Ihara, Masaki | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.jpkaWUyV.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Ogita, Tasuku | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.yhcePhkh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Shimosato, Takeshi | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html | |||||
出版者 | ||||||
出版者 | SPRINGER | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 101(1):341-349 (2017) | |||||
書誌情報 |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 巻 101, 号 1, p. 341-349, 発行日 2017-01 |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Epub 2016 Oct 8. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Interleukin 6 (IL-6) is an important pathogenic factor in development of various inflammatory and autoimmune diseases and cancer. Blocking antibodies against molecules associated with IL-6/IL-6 receptor signaling are an attractive candidate for the prevention or therapy of these diseases. In this study, we developed a genetically modified strain of Lactococcus lactis secreting a single-chain variable fragment antibody against mouse IL-6 (IL6scFv). An IL6scFv-secretion vector was constructed by cloning an IL6scFv gene fragment into a lactococcal secretion plasmid and was electroporated into L. lactis NZ9000 (NZ-IL6scFv). Secretion of recombinant IL6scFv (rIL6scFv) by nisin-induced NZ-IL6scFv was confirmed by western blotting and was optimized by tuning culture conditions. We found that rIL6scFv could bind to commercial recombinant mouse IL-6. This result clearly demonstrated the immunoreactivity of rIL6scFv. This is the first study to engineer a genetically modified strain of lactic acid bacteria (gmLAB) that produces a functional anti-cytokine scFv. Numerous previous studies suggested that mucosal delivery of biomedical proteins using gmLAB is an effective and low-cost way to treat various disorders. Therefore, NZ-IL6scFv may be an attractive tool for the research and development of new IL-6 targeting agents for various inflammatory and autoimmune diseases as well as for cancer. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0175-7598 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10503386 | |||||
PubMed | ||||||
識別子タイプ | PMID | |||||
関連識別子 | https://pubmed.ncbi.nlm.nih.gov/27722916/ | |||||
関連名称 | 27722916 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1007/s00253-016-7907-8 | |||||
関連名称 | 10.1007/s00253-016-7907-8 | |||||
権利 | ||||||
権利情報 | The original publication is available at www.springerlink.com | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |