2024-03-29T14:47:39Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00003670
2022-12-14T04:40:07Z
461:462
Heart-type fatty-acid-binding protein (FABP3) is a lysophosphatidic acid-binding protein in human coronary artery endothelial cells
Tsukahara, Ryoko
Haniu, Hisao
Matsuda, Yoshikazu
Tsukahara, Tamotsu
Copyright©2014 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. This<br/>is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).
FABP3
Lysophosphatidic acid
HCAEC
Peroxisome proliferator-activated receptor
gamma
Fatty-acid-binding protein 3, muscle and heart (FABP3), also known as heart-type FABP, is a member of the family of intracellular lipid-binding proteins. It is a small cytoplasmic protein with a molecular mass of about 15 kDa. FABPs are known to be carrier proteins for transporting fatty acids and other lipophilic substances from the cytoplasm to the nucleus, where these lipids are released to a group of nuclear receptors such as peroxisome proliferator-activated receptors (PPARs). In this study, using lysophosphatidic acid (LPA)-coated agarose beads, we have identified FABP3 as an LPA carrier protein in human coronary artery endothelial cells (HCAECs). Administration of LPA to HCAECs resulted in a dose-dependent increase in PPARγ activation. Furthermore, the LPA-induced PPARγ activation was abolished when the FABP3 expression was reduced using small interfering RNA (siRNA). We further show that the nuclear fraction of control HCAECs contained a significant amount of exogenously added LPA, whereas FABP3 siRNA-transfected HCAECs had a decreased level of LPA in the nucleus. Taken together, these results suggest that FABP3 governs the transcriptional activities of LPA by targeting them to cognate PPARγ in the nucleus.
Article
FEBS Open Bio. 4:947-951 (2014)
ELSEVIER SCIENCE LONDON
2014
eng
journal article
VoR
http://hdl.handle.net/10091/18035
https://soar-ir.repo.nii.ac.jp/records/3670
https://pubmed.ncbi.nlm.nih.gov/25426414
25426414
https://doi.org/10.1016/j.fob.2014.10.014
10.1016/j.fob.2014.10.014
2211-5463
FEBS Open Bio
4
947
951
https://soar-ir.repo.nii.ac.jp/record/3670/files/Heart-type_fatty-acid-binding_protein.pdf
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2015-09-24