2024-03-29T06:10:31Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00003679
2022-12-14T04:07:32Z
461:462
A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min
Uehara, Masayuki
Matsuda, Kazuyuki
Sugano, Mitsutoshi
Honda, Takayuki
Copyright© 2014 The Japanese Society of Veterinary Science
bovine respiratory syncytial virus
domestic animal
droplet-real-time PCR
infectious disease
The polymerase chain reaction (PCR) has been widely used for diagnosis of infectious diseases of domestic animals. Rapid detection of respiratory pathogens of cattle is useful for making therapeutic decisions. Therefore, we developed a new genetic-based method called droplet-real-time PCR, which can detect bovine respiratory syncytial virus (BRSV) within 10 min. Our droplet-real-time PCR markedly reduced the reaction time of reverse transcription-PCR while maintaining the same sensitivity as conventional real-time PCR, and it can be used as a rapid assay for detection of BRSV. Furthermore, our method is potentially applicable for rapid diagnosis of almost all infectious diseases, including highly pathogenic avian influenza virus.
Article
JOURNAL OF VETERINARY MEDICAL SCIENCE. 76(3):477-480 (2014)
JAPAN SOC VET SCI
2014-03
eng
journal article
VoR
http://hdl.handle.net/10091/17830
https://soar-ir.repo.nii.ac.jp/records/3679
https://pubmed.ncbi.nlm.nih.gov/24285011
24285011
https://doi.org/10.1292/jvms.13-0357
10.1292/jvms.13-0357
0916-7250
AA10796138
JOURNAL OF VETERINARY MEDICAL SCIENCE
76
3
477
480
https://soar-ir.repo.nii.ac.jp/record/3679/files/New_High-Speed_Droplet-Real-Time_Polymerase_Chain.pdf
application/pdf
1.5 MB
2015-09-24