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Culture medium type affects endocytosis of multi-walled carbon nanotubes in BEAS-2B cells and subsequent biological response
Haniu, Hisao
Saito, Naoto
Matsuda, Yoshikazu
Tsukahara, Tamotsu
Maruyama, Kayo
Usui, Yuki
Aoki, Kaoru
Takanashi, Seiji
Kobayashi, Shinsuke
Nomura, Hiroki
Okamoto, Masanori
Shimizu, Masayuki
Kato, Hiroyuki
Copyright© 2013 The Authors. Published by Elsevier Ltd. / This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Multi-walled carbon nanotube
BEAS-2B cells
Normal human bronchial epithelial cells
Cytotoxicity
Endocytosis
We examined the cytotoxicity of multi-walled carbon nanotubes (MWCNTs) and the resulting cytokine secretion in BEAS-2B cells or normal human bronchial epithelial cells (HBEpCs) in two types of culture media (Ham’s F12 containing 10% FBS [Ham’s F12] and serum-free growth medium [SFGM]). Cellular uptake of MWCNT was observed by fluorescent microscopy and analyzed using flow cytometry. Moreover, we evaluated whether MWCNT uptake was suppressed by 2 types of endocytosis inhibitors. We found that BEAS-2B cells cultured in Ham’s F12 and HBEpCs cultured in SFGM showed similar biological responses, but BEAS-2B cells cultured in SFGM did not internalize MWCNTs, and the 50% inhibitory concentration value, i.e., the cytotoxicity, was increased by more than 10-fold. MWCNT uptake was suppressed by a clathrin-mediated endocytosis inhibitor and a caveolae-mediated endocytosis inhibitor in BEAS-2B cells cultured in Ham’s F12 and HBEpCs cultured in SFGM. In conclusion, we suggest that BEAS-2B cells cultured in a medium containing serum should be used for the safety evaluation of nanomaterials as a model of normal human bronchial epithelial cells. However, the culture medium composition may affect the proteins that are expressed on the cytoplasmic membrane, which may influence the biological response to MWCNTs.
Article
Toxicology in Vitro. 27(6):1679-1685 (2013)
Elsevier
2013-09
eng
journal article
VoR
http://hdl.handle.net/10091/17038
https://soar-ir.repo.nii.ac.jp/records/3780
https://pubmed.ncbi.nlm.nih.gov/23648666
23648666
https://doi.org/10.1016/j.tiv.2013.04.012
10.1016/j.tiv.2013.04.012
0887-2333
AA10678594
Toxicology in Vitro
27
6
1679
1685
https://soar-ir.repo.nii.ac.jp/record/3780/files/Culture_medium_type_affects_endocytosis_multi-walled_carbon_nanotubes.pdf
application/pdf
1.3 MB
2015-09-24