2024-03-29T10:03:07Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00003791
2022-12-14T04:40:18Z
461:462
Selective alpha(1A)-Adrenoceptor Stimulation Induces Mueller's Smooth Muscle Contraction in an Isolated Canine Upper Eyelid Preparation
Yano, Shiharu
Hirose, Masamichi
Nakada, Tsutomu
Nakayama, Jun
Matsuo, Kiyoshi
Yamada, Mitsuhiko
Copyright© 2010 Informa Healthcare USA, Inc.
alpha(1)-Adrenoceptors
Blepharoptosis
Canine
Eyelid
Mueller's smooth muscle
Purpose: It has been demonstrated that in patients with aponeurotic blepharoptosis, alpha(1)-adrenoceptor stimulation causes the contraction of the upper eyelid tarsal smooth muscle (Mueller's muscle) and opening of the eye. However, alpha(1)-adrenoceptor subtypes mediating the contraction of Mueller's muscle are still unclear. This study was designed to identify the alpha(1)-adrenoceptor subtypes in Mueller's muscle. Materials and Methods: A newly developed canine upper eyelid preparation was retrogradely perfused with a drug-containing Krebs-Henseleit solution through the angular vein in a temperature-controlled organ chamber. The contraction of the preparation was measured with a force-displacement transducer. Results: Phenylephrine, an alpha(1)-adrenoceptor agonist, increased the upper eyelid contractile force in a dose-dependent manner (K(0.5) = 110 nmol). Interestingly, the contraction in response to phenylephrine was persistent and hardly recovered to a base line level for more than 100 min after washout of the drug. WB4101 (100 nM), an alpha(1A)- and alpha(1D)-adrenoceptor antagonist, but not BMY7378 (100 nM), a selective alpha(1D)-adrenoceptor antagonist, competitively inhibited the phenylephrine-induced contraction. ABT-866, a selective alpha(1A)-adrenoceptor agonist, increased the upper eyelid contractile force as effectively as phenylephrine in a dose-dependent manner (K(0.5) = 190 nmol), and the contraction continued again for more than 100 min. Conclusion: These results suggest that selective alpha(1A)-adrenoceptor agonists, such as ABT-866, induce the sustained Mueller's muscle contraction and may be useful in pharmacological treatment of blepharoptosis.
Article
CURRENT EYE RESEARCH. 35(5):363-369 (2010)
INFORMA HEALTHCARE
2010-05
eng
journal article
AM
http://hdl.handle.net/10091/16900
https://soar-ir.repo.nii.ac.jp/records/3791
https://pubmed.ncbi.nlm.nih.gov/20450248
20450248
https://doi.org/10.3109/02713680903518858
10.3109/02713680903518858
0271-3683
AA10621338
CURRENT EYE RESEARCH
35
5
363
369
https://soar-ir.repo.nii.ac.jp/record/3791/files/Selective_a1A-Adrenoceptor_Stimulation_Induces_Mueller’s_Smooth_Muscle_Contraction.pdf
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