2024-03-29T15:17:17Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00003850
2022-12-14T04:39:58Z
461:462
Genes for Difference in Eosinophilic Phenotype between MES and BN.MES-Cyba(mes) Rats Are on Chromosomes 9, 5, and 1
Tomozawa, Hiroshi
Nishio, Ayako
Higuchi, Keiichi
Matsumoto, Kiyoshi
Mori, Masayuki
Copyright (c) 2011 Japanese Association for Laboratory Animal Science
Cyba
eosinophilia
genetic polymorphism
NADPH oxidase
rat strain
The Matsumoto Eosinophilia Shinshu (MES) rat strain develops hereditary blood eosinophilia due to the mutant Cyba(mes) gene. In contrast, BN.MES-Cyba(mes) congenic rats, in which the mutant Cyba(mes) gene introduced into the background of the BN strain, have a normal blood eosinophil level despite showing robust proliferation of eosinophils in the bone marrow. However, the congenic rats manifest focal necrosis with eosinophilic infiltration in the liver, a phenotype rarely observed in the original MES rat strain. To elucidate the genetic basis for the strain differences, (MES x BN.MES-Cyba(mes))F(2) rats were bred, and genetic analyses of phenotypes for eosinophilia were performed. Blood and bone marrow eosinophil levels in the F(2) rats showed broad distributions, suggesting that the traits were under the influence of multiple genes. Genetic association studies revealed that BN-derived marker loci on chromosomes 9 and 5 were responsible for the increase in eosinophil level in the bone marrow, decrease in blood eosinophil level, and the induction of focal necrosis with eosinophilic infiltration in the liver. The BN-derived allele of the marker gene on chromosome 1 was responsible for the decrease of both bone marrow and blood eosinophil levels. These data suggest the existence of genes characterizing/distinguishing the eosinophilic phenotypes of MES and BN.MES-Cyba(mes) on these chromosomes, and form the basis for positional cloning studies of the genes. These studies will advance the understanding of the mechanisms involved in eosinophil mobilization from the bone marrow and recruitment to the organs.
Article
EXPERIMENTAL ANIMALS. 60(2):151-160 (2011)
Japanese Association for Laboratory Animal Science
2011-04
eng
journal article
VoR
http://hdl.handle.net/10091/13266
https://soar-ir.repo.nii.ac.jp/records/3850
https://pubmed.ncbi.nlm.nih.gov/21512270
21512270
https://doi.org/10.1538/expanim.60.151
10.1538/expanim.60.151
1341-1357
AA11032321
EXPERIMENTAL ANIMALS
60
2
151
160
https://soar-ir.repo.nii.ac.jp/record/3850/files/Genes_Difference_Eosinophilic_Phenotype.pdf
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