2024-03-28T10:22:51Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00010274
2022-12-14T04:30:21Z
1016:1017
Establishment and characterization of mouse bone marrow-derived mast cell hybridomas
Kawahara, Takeshi
Copyright© 2012 Elsevier Inc. All rights reserved.
Mast cell
Cell fusion
P815
Hybridoma
Degranulation
Toll-like receptor
Interleukin (IL)-3-dependent mouse bone marrow-derived mast cells (BMMCs) are an important model for studying the function of mucosal-type mast cells. In the present study, BMMCs were successfully immortalized by cell fusion using a hypoxanthine-aminopterin-thymidine medium-sensitive variant of P815 mouse mastocytoma (P815-6TgR) as a partner cell line. The established mouse mast cell hybridomas (MMCHs) expressed alpha, beta, and gamma subunits of high-affinity immunoglobulin E (IgE) receptor (FcERI) and possessed cytoplasmic granules devoid of or partially filled with electron-dense material. Four independent MMCH clones continuously proliferated without supplemental exogenous IL-3 and showed a degranulation response on stimulation with IgE+antigen. Furthermore, histamine synthesis and release by degranulation were confirmed in MMCH-D5, a MMCH clone that showed the strongest degranulation response. MMCH-D5 exhibited elevated levels of 1L-3, IL-4, IL-13, granulocyte-macrophage colony-stimulating factor, tumor necrosis factor (TNF)-alpha, and cyclooxygenase 2, and production of prostaglandin D2 and leukotriene C4 in response to IgE-induced stimulation. MMCH clones also expressed Toll-like receptors (TLRs) 1, 2, 4, and 6 and showed elevated levels of TNF-alpha expression in response to stimulation with TLR2 and TLR4 ligands. The MMCHs established using this method should be suitable for studies on FcERI- and TLR-mediated effector functions of mast cells.
Article
EXPERIMENTAL CELL RESEARCH. 318(18):2385-2396 (2012)
ELSEVIER INC
2012-11
eng
journal article
AM
http://hdl.handle.net/10091/17107
https://soar-ir.repo.nii.ac.jp/records/10274
https://pubmed.ncbi.nlm.nih.gov/22824512/
22824512
https://doi.org/10.1016/j.yexcr.2012.07.010
10.1016/j.yexcr.2012.07.010
0014-4827
AA00641011
EXPERIMENTAL CELL RESEARCH
318
18
2385
2396
https://soar-ir.repo.nii.ac.jp/record/10274/files/Establishment_characterization_mouse_bone_marrow-derived_mast_cell_hybridomas.pdf
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2015-09-25