2024-03-29T01:16:01Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00010581
2023-01-06T04:21:38Z
1016:1018:1019:1037
Effect of Ketonic Organic Solvents on Thermolysin: A Spectrophotometric Approach
サーモリシンに及ぼすケトン性有機溶媒の影響:紫外スペクトル測定分析
Alam, Nurul M
Tadasa, K
Kayahara, H
thermolysin
ketone
ultraviolet spectra
hypochromic effect
bathochromic (red) shift
Ultraviolet spectra of thermolysin on mixing with n-alkyl ketones were investigated. Hypochromic effect of UV spectra of enzyme and model compounds was observed. A clear batho-chromic shift (red shift) of thermolysin spectrum was also demonstrated. With increase in chain length of ketones, the red shift was found reduced. The hypochromic and bathochromic effects were significantly suppressed when enzyme or model compounds were pre-treated with inhibitor (phosphor-amidon). There had been no such effect of ketones upon mixing with substrates. It was speculated that ketones interact with the UV sensitive aromatic amino acid residues at the microenvironment of active site of enzyme.
Article
信州大学農学部紀要. 34(1): 31-37 (1997)
信州大学農学部
1997-09-25
eng
journal article
VoR
http://hdl.handle.net/10091/885
https://soar-ir.repo.nii.ac.jp/records/10581
0583-0621
AN00121352
信州大学農学部紀要
34
1
31
37
https://soar-ir.repo.nii.ac.jp/record/10581/files/v34n01_05.pdf
application/pdf
380.5 kB
2015-09-25