2024-03-29T06:18:46Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00012147
2023-03-08T01:47:13Z
1169:1171:1172:1200
ギンブナ卵ホモジェネートによる精子核クロマチンの拡散
In vitro analysis on the decondensation of sperm head clomatin by the homogenates of oocyte cytoplasm in Carassius auratus langsdorfii
松村, 清隆
梶島, 孝雄
Sperm chromatin decondensing activity of funa eggs and oocytes was studied in vitro, in order to clarify the mechanism of sperm chromatin decondensation in egg cytoplasm. To assay for sperm chromatin decondensing activity, naked sperm nuclei treated with Triton were exposed to homogenate from oocytes and eggs. As a result of experiments using funa sperm, full-grown oocytes of diploid funa induced chromatin decondensation, though growing oocytes, matured eggs of diploid, and oocytes and matured eggs of triploid did not. On the other hand, using medaka sperm, fullgrown oocytes of triploid funa also induced chromatin decondensation. From the present study, it becomes apparent that homogenate from full-grown oocytes before germinal vesicle breakdown possess the ability to decondense chromatin. And it seems to be probable that egg cytoplasm of triploid ginbuna does not lack the ability to decondense chromatin but possesses the factor(s) inhibiting decondensation of sperm chromatin of the other sub-species.
Article
信州大学理学部紀要 19(1): 63-72(1984)
信州大学理学部
1984-10-15
jpn
departmental bulletin paper
VoR
http://hdl.handle.net/10091/10546
https://soar-ir.repo.nii.ac.jp/records/12147
0583-063X
AA00697923
信州大学理学部紀要
19
1
63
72
https://soar-ir.repo.nii.ac.jp/record/12147/files/Science19-01-06.pdf
application/pdf
954.8 kB
2015-09-28