2024-03-29T00:03:21Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00014487
2022-12-14T04:05:05Z
1309:1314:1458:1461
15-3-10 : 細胞内繊維構造体の分子集合を利用したマイクロマシーンの創出
生体材料を用いたバイオミメティックス材料の開発
藤井, 敏弘
平井, 利博
Isolation of biopolymers, tissues, and cells from the host body system is necessary to protect the implanted polymers from protease and immune systems. Encapsulation technology is thought to be an effective for solving these problems, and further can be used in bioreactor and biosensor fields. Muscle proteins such as myosin, action, and actomyosin were entrapped in gellan/chitosan polyion complex (PIC) capsules using a simple procedure. The incorporation of muscle proteins was examined by ATP hydrolysis and electrophoresis. The recoveries of myosin and actomyosin ATPase activities were approximately 30% and 35%, respectively, when the capsules were prepared by dropping a mixture solution of the proteins and gellan into a chitosan solution. The activation of myosin Mg2+-ATPase activity, by actin was also observed after their incorporation into the capsules. Myosin in the capsules mainly consisted of heavy chain and light chains without remarkable cleavage. These results suggest that this system will be an excellent model for contractile apparatus.
Article
先進ファイバー工学研究教育拠点研究成果報告書 11: 78-78(2005)
信州大学繊維学部
2005-03-31
jpn
research report
VoR
http://hdl.handle.net/10091/13475
https://soar-ir.repo.nii.ac.jp/records/14487
AA1200368X
先進ファイバー工学研究教育拠点研究成果報告書
11
78
78
https://soar-ir.repo.nii.ac.jp/record/14487/files/AFSTT11-309.pdf
application/pdf
159.3 kB
2015-09-28