2024-03-28T11:06:03Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00014582
2022-12-14T04:05:12Z
1309:1314:1458:1462
15-2-8 :キチン・キトサンの酵素変換による高機能化に関する研究
天然繊維の高機能化と応用
下坂, 誠
小平, 律子
A gene (csnC) coding for an extracellular chitosaanse was isolated from the koji mold, Aspergillus oryzae, and a complete nucleotide sequence was determined. Chitosanase cDNA derived from the fungus Fusarium solani was expressed in Saccharomyces cerevisiae. By using this expression system, two amino acid residues responsible for catalysis were determined, and an inverting-type reaction mechanism was also confirmed. A group of genes specifically expressed at the stage of fruit body development was isolated from the basidiomycete Flammulina velutipes. One of the genes encoded a polypeptide having a primary sequence similar to those of polysaccharide deacetylases. A metagenome Library was constructed from choromosomal DNAs prepared from whole bacterial cells in soil. From this library, E. coli transformant exhibiting a red fluorescent light was isolated and a gene structure in the cloned DNA fragment was analyzed.
Article
先進ファイバー工学研究教育拠点研究成果報告書 10: 52-53(2004)
信州大学繊維学部
2004-03-31
jpn
research report
VoR
http://hdl.handle.net/10091/13561
https://soar-ir.repo.nii.ac.jp/records/14582
AA1200368X
先進ファイバー工学研究教育拠点研究成果報告書
10
52
53
https://soar-ir.repo.nii.ac.jp/record/14582/files/AFSTT10-208.pdf
application/pdf
739.0 kB
2015-09-28