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2022-12-14T04:27:27Z
1309:1310
Disruption of diapause induction by TALEN-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in Bombyx
Shiomi, Kunihiro
Takasu, Yoko
Kunii, Masayo
Tsuchiya, Ryoma
Mukaida, Moeka
Kobayashi, Masakazu
Sezutsu, Hideki
Ichida, (Takahama), Masatoshi
Mizoguchi, Akira
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The insect neuropeptide family FXPRLa, which carries the Phe-Xaa-Pro-Arg-Leu-NH2 sequence at the C-terminus, is involved in many physiological processes. Although ligand–receptor interactions in FXPRLa signaling have been examined using in vitro assays, the correlation between these interactions and in vivo physiological function is unclear. Diapause in the silkworm, Bombyx mori, is thought to be elicited by diapause hormone (DH, an FXPRLa) signaling, which consists of interactions between DH and DH receptor (DHR). Here, we performed transcription activator-like effector nuclease (TALEN)-based mutagenesis of the Bombyx DH-PBAN and DHR genes and isolated the null mutants of these genes in a bivoltine strain. All mutant silkworms were fully viable and showed no abnormalities in the developmental timing of ecdysis or metamorphosis. However, female adults oviposited non-diapause eggs despite diapause-inducing temperature and photoperiod conditions. Therefore, we conclude that DH signaling is essential for diapause induction and consists of highly sensitive and specific interactions between DH and DHR selected during ligand–receptor coevolution in Bombyx mori.
Article
SCIENTIFIC REPORTS. 5:15566 (2015)
NATURE PUBLISHING GROUP
2015-10-26
eng
journal article
VoR
http://hdl.handle.net/10091/00018689
https://soar-ir.repo.nii.ac.jp/records/17921
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=26497859
26497859
https://doi.org/10.1038/srep15566
10.1038/srep15566
2045-2322
SCIENTIFIC REPORTS
5
15566
https://soar-ir.repo.nii.ac.jp/record/17921/files/Disruption_of_diapause_induction_by_TALEN-based_gene.pdf
application/pdf
2.3 MB
2016-03-23