2024-03-29T10:41:03Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00019064
2022-12-14T03:55:28Z
461:462
Diversity and Complexity of the Mouse Saa1 and Saa2 genes
Mori, Masayuki
Tian, Geng
Ishikawa, Akira
Higuchi, Keiichi
© 2014 Japanese Association for Laboratory Animal Science
gene conversion
gene duplication
Mus subspecies
polymorphism
serum amyloid A
Mouse strains show polynnorphisms in the amino acid sequences of serum amyloid A 1 (SAA1) and serum amyloid A 2 (SAA2). Major laboratory mouse strains are classified based on the sequence as carrying the A haplotype (e.g., BALB/c) or B haplotype (e.g., SJL/J) of the Seal and Saa2 gene unit. We attempted to elucidate the diversity of the mouse Saa1 and Saa2 family genes at the nucleotide sequence level by a systematic survey of 6 inbred mouse strains from 4 Mus subspecies, including Mus musculus domesticus, Mus musculus musculus, Mus musculus castaneus, and Mus spretus. Saa1 and Saa2 genes were obtained from the mouse genome by PCR amplification, and each full-length nucleotide sequence was determined. We found that Mus musculus castaneus mice uniquely possess 2 divergent Saa1 genes linked on chromosome 7. Overall, the mouse strains had distinct composite patterns of amino acid substitutions at 9 positions in SAA1 and SAA2 isoforms. The mouse strains also had distinct composite patterns of 2 polymorphic upstream regulatory elements that influenced gene transcription in in vitro reporter assays. B haplotype mice were revealed to possess an LTR insertion in the downstream region of Saa1. Collectively, these results indicate that the mouse Saa genes hold broader diversity and greater complexity than previously known, and these characteristics were likely attained through gene duplication and repeated gene conversion events in the Mus lineage.
Article
EXPERIMENTAL ANIMALS. 63(1):99-106 (2014)
INT PRESS EDITING CENTRE INC
2014-01
eng
journal article
VoR
http://hdl.handle.net/10091/00019826
https://soar-ir.repo.nii.ac.jp/records/19064
https://pubmed.ncbi.nlm.nih.gov/24521869
24521869
https://doi.org/10.1538/expanim.63.99
10.1538/expanim.63.99
1341-1357
AA11032321
EXPERIMENTAL ANIMALS
63
1
99
106
https://soar-ir.repo.nii.ac.jp/record/19064/files/Diversity_and_Complexity_of_the_Mouse_Saa1_and_Saa2_genes.pdf
application/pdf
1.8 MB
2017-09-20