2024-03-29T05:30:20Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00020696
2022-12-14T04:30:31Z
1016:1017
Autonomous xenogenic cell fusion of murine and chick skeletal muscle myoblasts
Takaya, Tomohide
Nihashi, Yuma
Kojima, Shotaro
Ono, Tamao
Kagami, Hiroshi
© 2017 Japanese Society of Animal Science, This is the peer reviewed version of the following article: Anim Sci J, 88: 1880-1885, 2017, which has been published in final form at https://doi.org/10.1111/asj.12884 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.<br/>
cell fusion
hybrid cell
myotube formation
skeletal muscle myoblast
Cell-cell fusion has been a great technology to generate valuable hybrid cells and organisms such as hybridomas. In this study, skeletal muscle myoblasts were utilized to establish a novel method for autonomous xenogenic cell fusion. Myoblasts are mononuclear myogenic precursor cells and fuse mutually to form multinuclear myotubes. We generated murine myoblasts (mMBs) expressing green fluorescent protein (GFP) termed mMB-GFP, and the chick myoblasts (chMBs) expressing Discosoma red fluorescent protein (DsRed) termed chMB-DsRed. mMB-GFP and chMB-DsRed were cocultured and induced to differentiate. After 24h, the multinuclear myotubes expressing both GFP and DsRed were observed, indicating that mMBs and chMBs interspecifically fuse. These GFP(+)/DsRed(+) hybrid myotubes were able to survive and grew to hyper-multinucleated mature form. We also found that undifferentiated mMB-GFP efficiently fuse to the chMB-DsRed-derived myotubes. This is the first evidence for the autonomous xenogenic fusion of mammalian and avian cells. Myoblast-based fusogenic technique will open up an alternative direction to create novel hybrid products.
Article
ANIMAL SCIENCE JOURNAL. 88(11):1880-1885 (2017)
WILEY
2017-11
eng
journal article
AM
http://hdl.handle.net/10091/00021454
https://soar-ir.repo.nii.ac.jp/records/20696
https://pubmed.ncbi.nlm.nih.gov/28782148/
28782148
https://doi.org/10.1111/asj.12884
10.1111/asj.12884
1344-3941
AA11677214
ANIMAL SCIENCE JOURNAL
88
11
1880
1885
https://soar-ir.repo.nii.ac.jp/record/20696/files/2017_AnimSciJ_Takaya_SOAR.pdf
application/pdf
1.3 MB
2019-05-08