2024-03-28T23:08:12Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:02000345
2022-12-14T04:41:17Z
461:462
Evaluation of MC3T3-E1 Cell Osteogenesis in Different Cell Culture Media
Izumiya, Makoto
Haniu, Miyu
Ueda, Katsuya
Ishida, Haruka
Ma, Chuang
Ideta, Hirokazu
Sobajima, Atsushi
Ueshiba, Koki
Uemura, Takeshi
Saito, Naoto
Haniu, Hisao
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
biomaterials
vitamin C
osteoblast
calcification
cell culture
collagen
alkaline phosphatase
Many biomaterials have been evaluated using cultured cells. In particular, osteoblast-like cells are often used to evaluate the osteocompatibility, hard-tissue-regeneration, osteoconductive, and osteoinductive characteristics of biomaterials. However, the evaluation of biomaterial osteogenesis-inducing capacity using osteoblast-like cells is not standardized; instead, it is performed under laboratory-specific culture conditions with different culture media. However, the effect of different media conditions on bone formation has not been investigated. Here, we aimed to evaluate the osteogenesis of MC3T3-E1 cells, one of the most commonly used osteoblast-like cell lines for osteogenesis evaluation, and assayed cell proliferation, alkaline phosphatase activity, expression of osteoblast markers, and calcification under varying culture media conditions. Furthermore, the various media conditions were tested in uncoated plates and plates coated with collagen type I and poly-L-lysine, highly biocompatible molecules commonly used as pseudobiomaterials. We found that the type of base medium, the presence or absence of vitamin C, and the freshness of the medium may affect biomaterial regeneration. We posit that an in vitro model that recapitulates in vivo bone formation should be established before evaluating biomaterials.
Article
International Journal of Molecular Sciences. 22(14):7752 (2021)
MDPI
2021-07-20
eng
journal article
VoR
http://hdl.handle.net/10091/0002000345
https://soar-ir.repo.nii.ac.jp/records/2000345
https://pubmed.ncbi.nlm.nih.gov/34299372/
34299372
https://doi.org/10.3390/ijms22147752
10.3390/ijms22147752
1422-0067
International Journal of Molecular Sciences
22
14
7752
https://soar-ir.repo.nii.ac.jp/record/2000345/files/ijms-22-07752-v3.pdf
application/pdf
4.7 MB
2021-07-28