2024-03-28T12:28:19Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:02000388
2022-12-14T04:28:40Z
1309:1310
Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum (common buckwheat)
Koja, Eiki
Ohata, Soichiro
Maruyama, Yoshinori
Suzuki, Hideyuki
Shimosaka, Makoto
Taguchi, Goro
common buckwheat
flavonoid biosynthesis
rhamnosyltransferase
rutin
Rutin, a 3-rutinosyl quercetin, is a representative flavonoid distributed in many plant species, and is highlighted for its therapeutic potential. In this study, we purified uridine diphosphate-rhamnose: quercetin 3-O-glucoside 6″-O-rhamnosyltransferase and isolated the corresponding cDNA (FeF3G6″RhaT) from seedlings of common buckwheat (Fagopyrum esculentum). The recombinant FeF3G6″RhaT enzyme expressed in Escherichia coli exhibited 6″-O-rhamnosylation activity against flavonol 3-O-glucoside and flavonol 3-O-galactoside as substrates, but showed only faint activity against flavonoid 7-O-glucosides. Tobacco cells expressing FeF3G6″RhaT converted the administered quercetin into rutin, suggesting that FeF3G6″RhaT can function as a rhamnosyltransferase in planta. Quantitative PCR analysis on several organs of common buckwheat revealed that accumulation of FeF3G6″RhaT began during the early developmental stages of rutin-accumulating organs, such as flowers, leaves, and cotyledons. These results suggest that FeF3G6″RhaT is involved in rutin biosynthesis in common buckwheat.
Bioscience, Biotechnology, and Biochemistry. 82(10):1790–1802(2018)
Japan Society for Bioscience, Biotechnology, and Agrochemistry
2018-10-03
eng
journal article
AM
http://hdl.handle.net/10091/0002000388
https://soar-ir.repo.nii.ac.jp/records/2000388
https://pubmed.ncbi.nlm.nih.gov/29972345
https://doi.org/10.1080/09168451.2018.1491286
1347-6947
AA12018610
Bioscience, Biotechnology, and Biochemistry
82
10
1790
1802
https://soar-ir.repo.nii.ac.jp/record/2000388/files/1491286.pdf
application/pdf
2.9 MB
2021-09-28