2024-03-28T20:28:43Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00003802
2022-12-14T04:12:15Z
461:462
Freeze-thawing single human embryonic stem cells induce e-cadherin and actin filament network disruption via g13 signaling
Ichikawa, Hinako
Yoshie, Susumu
Shirasawa, Sakiko
Yokoyama, Tadayuki
Yue, Fengming
Tomotsune, Daihachiro
Sasaki, Katsunori
Cryopreservation
E-cadherin
actin filaments
G13 signaling pathway
human embryonic stem cells
ROCK inhibitor
Poor adhesion of single human embryonic stem (hES) cells after freeze-thawing causes death. To investigate mechanisms responsible for this, Rho-dependent protein kinase (ROCK) inhibitor Y-27632-treated and untreated single hES cells were analyzed for E-cadherin and F-actin distribution by immunostaining and phalloidin staining respectively and for G13 signaling pathway components by DNA microarray and quantitative polymerase chain reaction (PCR). Y-27632-treated cells clustered rapidly and maintained E-cadherin and F-actin distribution without losing Oct3/4. Immediately after thawing, E-cadherin in untreated hES cells dotted along the membrane and then displayed eccentric cytoplasmic localization. Bleb formation and early Oct3/4 loss occurred after F-actin network condensation in the cytoplasm. Microarray analyses and quantitative PCR indicated upregulation of two actin reorganization-associated components of the G13 signaling pathway, Arhgdib and Cdc42, in untreated cells. Considering these findings and that cell death was partly interrupted by Y-27632, E-cadherin and actin cytoskeleton network disruption through the G13 signaling pathway may cause hES cell death after freeze-thawing.
Article
CRYO LETTERS. 32(6):516-524 (2011)
journal article
CRYO LETTERS
2011
application/pdf
CRYO LETTERS
6
32
516
524
0143-2044
AA00145687
https://soar-ir.repo.nii.ac.jp/record/3802/files/Cryoletters32.6.pp516-524.pdf
eng
http://www.cryoletters.org/
http://www.cryoletters.org/
22227712
https://pubmed.ncbi.nlm.nih.gov/22227712
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