2024-03-28T17:37:27Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00003830
2022-12-14T04:39:55Z
461:462
Exercise Effects on Methylation of ASC Gene
Nakajima, K
Takeoka, M
Mori, M
Hashimoto, S
Sakurai, A
Nose, H
Higuchi, K
Itano, N
Shiohara, M
Oh, T
Taniguchi, S
interval walking
pyrosequencing
age
Chronic moderate exercise has been reported to reduce pro-inflammatory cytokines. To analyze the molecular mechanisms by which training exerts these effects, the epigenetic influences of age and exercise on the ASC gene, which is responsible for IL-1 beta and IL-18 secretion, were investigated by ASC gene methylation. Further, the relationship between carcinogenesis and exercise, and methylation of the p15 tumor suppressive gene was also analyzed. High-intensity interval walking exercise, consisting of 3 min low-intensity walking at 40% of peak aerobic capacity followed by a 3 min high-intensity walking period above 70% of peak aerobic capacity, was continued for 6 months. Peripheral blood DNA extracts from young control (n = 34), older control (n = 153), and older exercise (n = 230) groups were then analyzed by pyrosequencing for DNA methylation. Methylation of ASC decreased significantly with age (young control vs. older control, p < 0.01), which is indicative of an age-dependent increase in ASC expression. Compared to the older control group, the degree of ASC methylation was higher in the older exercise group (older control vs. older exercise: p < 0.01), and presumably lower ASC expression. Neither exercise nor age affected the methylation of the p15. In summary, chronic moderate exercise appears to attenuate the age-dependent decrease in ASC methylation, implying suppression of excess pro-inflammatory cytokines through reduction of ASC expression.
Article
INTERNATIONAL JOURNAL OF SPORTS MEDICINE. 31(9):671-675 (2010)
journal article
GEORG THIEME VERLAG KG
2010-09
application/pdf
INTERNATIONAL JOURNAL OF SPORTS MEDICINE
9
31
671
675
0172-4622
AA00234368
https://soar-ir.repo.nii.ac.jp/record/3830/files/Exercise_effects_methylation_ASC_gene.pdf
eng
20200803
https://pubmed.ncbi.nlm.nih.gov/20200803
10.1055/s-0029-1246140
https://doi.org/10.1055/s-0029-1246140
Copyright© 2010 Georg Thieme Verlag KG Stuttgart