2024-03-28T16:27:47Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00003900
2022-12-14T03:47:05Z
461:462
Analysis of plasmin generation and clot lysis of plasma fibrinogen purified from a heterozygous dysfibrinogenemia, B beta Gly15Cys (Hamamatsu II)
Kamijyo, Yuka
Hirota-Kawadobora, Masako
Terasawa, Fumiko
Honda, Takayuki
Ikeya, Manae
Okumura, Nobuo
albumin-binding form
B beta-chain
clot lysis
dysfibrinogen
infarction of the medulla oblongata
plasmin generation
This is a non-final version of an article published in final form in Blood Coagulation & Fibrinolysis. 20(8):726-732, December 2009.
We found a heterozygous dysfibrinogenemia caused by the substitution of B beta Gly15Cys and designated it fibrinogen Hamamatsu II (H-II). Although the propositus suffered an infarction of the medulla oblongata, other thrombotic risk factors, paradoxical cerebral infarction, and arterial dissection were not found. To determine whether the delayed lysis of fibrin clots or not in the context of the B beta Gly15Cys substitution, we examined the clot lysis and plasmin generation of propositus' fibrinogen. Fibrinogen was purified from the propositus' and normal control plasma by immunoaffinity chromatography and was used for the following experiments: sodium dodecyl sulfate-polyacrylamide gel electrophoresis, fibrin polymerization, scanning electron microscopic observation of fibrin clot and fibers, clot lysis, and tissue-type plasminogen activator-mediated plasminogen activation. The H-II plasma fibrinogen showed the presence of albumin-binding variant forms, a dimeric molecule of variant fibrinogen, and impairment of lateral aggregation during fibrin polymerization. The H-II fibrin clot showed lower density of bundles and thinner diameters of fibers than in the normal fibrin clot. In the clot lysis experiments with overlaid plasmin, H-II fibrin showed a similar lysis period and lysis rate to the normal control. Moreover, plasmin generation from a mixture of thrombin, tissue-type plasminogen activator, plasminogen, and H-II fibrinogen also showed a similar rate to normal fibrinogen. Although the propositus suffered an infarction, the present study did not observe delayed clot lysis, that is, the clot was not resistant to plasmin degradation. Therefore, we did not clarify an association between the B beta Gly15Cys dysfibrinogenemia and arterial thrombosis.
Article
BLOOD COAGULATION & FIBRINOLYSIS. 20(8):726-732 (2009)
journal article
LIPPINCOTT WILLIAMS & WILKINS
2009-12
application/pdf
BLOOD COAGULATION & FIBRINOLYSIS
8
20
726
732
0957-5235
AA10794937
https://soar-ir.repo.nii.ac.jp/record/3900/files/Analysis_plasmin_generation_clot_lysis_plasma_fibrinogen.pdf
eng
19809304
https://pubmed.ncbi.nlm.nih.gov/19809304
10.1097/MBC.0b013e328332aa09
https://doi.org/10.1097/MBC.0b013e328332aa09