2024-03-29T10:07:31Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00008028
2022-12-14T04:26:00Z
882:883
Mechanisms of CYP3A Induction by Glucocorticoids in Human Fetal Liver Cells
Matsunaga, Tamihide
Maruyama, Masataka
Matsubara, Tsutomu
Nagata, Kiyoshi
Yamazoe, Yasushi
Ohmori, Shigeru
CYP3A
induction
glucocorticoid
human fetal liver cells
glucocorticoid receptor
small interfering RNA
specific small interfering RNA
Human fetal liver (HFL) cells express major drug metabolic enzymes CYP3A4, CYP3A5 and CYP3A7. In the fetal hepatocytes, betamethasone and dexamethasone (DEX) markedly enhanced the expression levels of CYP3A4 and CYP3A7 mRNAs and slightly increased the expression level of CYP3A5 mRNA. Interestingly, a high correlation between the CYP3A induction ability and the intensity of anti-inflammatory effect was observed. Human glucocorticoid receptor (GR) small interfering RNA clearly attenuated the expression level of GR mRNA, and diminished the DEX-stimulated CYP3A4, CYP3A5 and CYP3A7 expression in HFL cells. These findings indicate that GR mediates the induction of CYP3A4 and CYP3A7 expression in human fetal hepatocytes as well as the CYP3A5.
Article
DRUG METABOLISM AND PHARMACOKINETICS. 27(6):653-657 (2012)
journal article
JAPANESE SOC STUDY XENOBIOTICS
2012-12
application/pdf
DRUG METABOLISM AND PHARMACOKINETICS
6
27
653
657
1347-4367
AA1162652X
https://soar-ir.repo.nii.ac.jp/record/8028/files/Mechanisms_CYP3A_Induction_Glucocorticoids_Human.pdf
eng
22673009
https://pubmed.ncbi.nlm.nih.gov/22673009
10.2133/dmpk.DMPK-12-NT-018
https://doi.org/10.2133/dmpk.DMPK-12-NT-018
Copyright© 2012 The Japanese Society for the Study of Xenobiotics