2024-03-29T09:30:48Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00008054
2022-12-14T04:00:44Z
882:883
Comparison of manual and digital microvascular density counting of RECK expression in glioma
Rahmah, Nunung Nur
Sakai, Keiichi
Li, Yuhui
Sano, Kenji
Hongo, Kazuhiro
digital image counting
glioma
microvascular density
RECK
A microvascular density (MVD) counting method for reversion-inducing cysteine-rich protein with Kazal motifs (RECK) expression, using a digital image analysis tool, has advantages over manual counting by microscope. Thirty glioma cases with RECK staining were photographed at a magnification of 200 x high power field and the photographs in RGB images were analyzed, and stained vessels were captured and were counted automatically. MVD with RECK expression using a digital image analysis tool showed comparable results to those of the manual method. RECK intensity expression could show linear correlation with grades of glioma by the digital method, which was superior compared to the manual method. The present method is recommended to researchers undertaking MVD study for glioma.
Article
NEUROPATHOLOGY. 32(3):245-251 (2012)
journal article
WILEY-BLACKWELL
2012-06
application/pdf
NEUROPATHOLOGY
3
32
245
251
0919-6544
AA11009964
https://soar-ir.repo.nii.ac.jp/record/8054/files/Comparison_manual_digital_microvascular_density_counting.pdf
eng
22098593
https://pubmed.ncbi.nlm.nih.gov/22098593
10.1111/j.1440-1789.2011.01265.x
https://doi.org/10.1111/j.1440-1789.2011.01265.x
Copyright© 2011 Japanese Society of Neuropathology