2024-03-29T11:07:46Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00010245
2022-12-14T04:02:09Z
1016:1017
Ultrastructural Study on Colocalization of Glucagon-Like Peptide (GLP)-1 with GLP-2 in Chicken Intestinal L-Cells
Nishimura, Kei
Hiramatsu, Kohzy
Monir, Mohammad M.
Takemoto, Chihiro
Watanabe, Takafumi
chicken
glucagon-like peptides
immunocytochemistry
immunohistochemistry
small intestine
Colocalization of glucagon-like peptide (GLP)-1 with GLP-2 in L-cells was investigated in the chicken ileum by using double immunofluorescent and immunocytochemical techniques. Ultrastructural features of L-cells were also clarified in this study. L-cells showing immunoreactivity for both GLP-1 and GLP-2 were distributed in the whole ileum. They showed comma-like or flask-like shape and were located in epithelium of crypts and lower part of intestinal villi. L-cells showing GLP-1-immunoreactivity only were found in epithelium of lower and middle parts of intestinal villi. Transmission electron microscopy indicated that L-cells identified by colloidal gold-labeled immunocytochemistry were covered apically with microvilli, open-type and contained many secretory granules in their perikarya. These secretory granules without halo were round to oval in shape and showed moderate electron density. The longest and shortest diameters of secretory granules were 355 +/- 62 nm (mean +/- SD) and 287 +/- 48 nm, respectively. Double labeling immunocytochemistry using two different sizes of particles (6 and 12 nm in diameter) of colloidal gold revealed that GLP-1 colocalized with GLP-2 in the same secretory granules. This study advances new morphological data about the endocrine system of the chicken small intestine.
Article
JOURNAL OF VETERINARY MEDICAL SCIENCE. 75(10):1335-1339 (2013)
journal article
JAPAN SOC VET SCI
2013-10
application/pdf
JOURNAL OF VETERINARY MEDICAL SCIENCE
10
75
1335
1339
0916-7250
AA10796138
https://soar-ir.repo.nii.ac.jp/record/10245/files/Ultrastructural_Study_Colocalization_Glucagon-Like_Peptide.pdf
eng
23759686
https://pubmed.ncbi.nlm.nih.gov/23759686/
10.1292/jvms.13-0106
https://doi.org/10.1292/jvms.13-0106
Copyright© 2013 The Japanese Society of Veterinary Science