2024-03-29T02:21:26Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00013341
2022-12-14T03:47:06Z
1309:1310
Recent Progress in Cryopreservation of Bovine Oocytes
Hwang, In-Sul
Hochi, Shinichi
Principle of oocyte cryoinjury is first overviewed and then research history of cryopreservation using bovine oocytes is summarized for the last two decades with a few special references to recent progresses. Various types of cryodevices have been developed to accelerate the cooling rate and applied to the oocytes from large domestic species enriched with cytoplasmic lipid droplets. Two recent approaches include the qualitative improvement of IVM oocytes prior to the vitrification and the short-term recovery culture of vitrified-warmed oocytes prior to the subsequent IVF. Supplementation of L-carnitine to IVM medium of bovine oocytes has been reported to reduce the amount of cytoplasmic lipid droplets and improve the cryotolerance of the oocytes, but it is still controversial whether the positive effect of L-carnitine is reproducible. Incidence of multiple aster formation, a possible cause for low developmental potential of vitrified-warmed bovine oocytes, was inhibited by a short-term culture of the postwarm oocytes in the presence of Rho-associated coiled-coil kinase (ROCK) inhibitor. Use of an antioxidant. alpha-tocopherol, instead of the ROCK inhibitor, also supported the revivability of the postwarm bovine oocytes. Further improvements of the vitrification procedure, combined with pre- and postvitrification chemical treatment, would overcome the high sensitivity of bovine oocytes to cryopreservation.
Article
BIOMED RESEARCH INTERNATIONAL. 2014:570647 (2014)
journal article
HINDAWI PUBLISHING CORPORATION
2014
application/pdf
BIOMED RESEARCH INTERNATIONAL
2014
:570647
2314-6133
https://soar-ir.repo.nii.ac.jp/record/13341/files/Recent_Progress_Cryopreservation_Bovine_Oocytes.pdf
eng
24738063
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=24738063
10.1155/2014/570647
https://doi.org/10.1155/2014/570647
Copyright© 2014 In-Sul Hwang and Shinichi Hochi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.