2024-03-28T21:08:36Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00014583
2022-12-14T04:05:11Z
1309:1314:1458:1462
天然繊維の高機能化と応用
15-2-9 :微生物酵素のよって合成される繊維状高分子に関する研究
志田, 敏夫
DNA fragment (tgl gene) for construction of Tgl (transglutaminase) expression plasmid was amplyfied by PCR (polymerase chain reaction) method using B. subtilis 168 chromosal DNA as atemplate. To obtain the His-tagged transglutaminase of B. subtilis, the fragment was cloned into the expression plasmid pQE30. Competent E. coli KP3998 was transformed with the resulting plasmid pQE-Tgl. The His-tagged Tgl protein was purified with His Trap column.
Article
先進ファイバー工学研究教育拠点研究成果報告書 10: 54-54(2004)
research report
信州大学繊維学部
2004-03-31
application/pdf
先進ファイバー工学研究教育拠点研究成果報告書
10
54
54
AA1200368X
https://soar-ir.repo.nii.ac.jp/record/14583/files/AFSTT10-209.pdf
jpn