2024-03-28T19:09:14Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00018586
2022-12-14T04:39:00Z
1016:1017
Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis
Shigemori, Suguru
Ihara, Masaki
Sato, Takashi
Yamamoto, Yoshinari
Nigar, Shireen
Ogita, Tasuku
Shimosato, Takeshi
Immunoreactivity
Interleukin 6
Lactococcus lactis
gmLAB
scFv antibody
Epub 2016 Oct 8.
Interleukin 6 (IL-6) is an important pathogenic factor in development of various inflammatory and autoimmune diseases and cancer. Blocking antibodies against molecules associated with IL-6/IL-6 receptor signaling are an attractive candidate for the prevention or therapy of these diseases. In this study, we developed a genetically modified strain of Lactococcus lactis secreting a single-chain variable fragment antibody against mouse IL-6 (IL6scFv). An IL6scFv-secretion vector was constructed by cloning an IL6scFv gene fragment into a lactococcal secretion plasmid and was electroporated into L. lactis NZ9000 (NZ-IL6scFv). Secretion of recombinant IL6scFv (rIL6scFv) by nisin-induced NZ-IL6scFv was confirmed by western blotting and was optimized by tuning culture conditions. We found that rIL6scFv could bind to commercial recombinant mouse IL-6. This result clearly demonstrated the immunoreactivity of rIL6scFv. This is the first study to engineer a genetically modified strain of lactic acid bacteria (gmLAB) that produces a functional anti-cytokine scFv. Numerous previous studies suggested that mucosal delivery of biomedical proteins using gmLAB is an effective and low-cost way to treat various disorders. Therefore, NZ-IL6scFv may be an attractive tool for the research and development of new IL-6 targeting agents for various inflammatory and autoimmune diseases as well as for cancer.
Article
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 101(1):341-349 (2017)
journal article
SPRINGER
2017-01
application/pdf
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
1
101
341
349
0175-7598
AA10503386
https://soar-ir.repo.nii.ac.jp/record/18586/files/Secretion_immunoreactive_single-chain_variable_fragment.pdf
eng
27722916
https://pubmed.ncbi.nlm.nih.gov/27722916/
10.1007/s00253-016-7907-8
https://doi.org/10.1007/s00253-016-7907-8
The original publication is available at www.springerlink.com