2024-03-28T12:59:50Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:00020084
2022-12-14T04:16:51Z
461:462
The Biphasic Effects of Oxidized-Low Density Lipoprotein on the Vasculogenic Function of Endothelial Progenitor Cells
Lin, FY
Tsao, NW
Shih, CM
Lin, YW
Yeh, JS
Chen, JW
Nakagami, H
Morishita, R
Sawamura, T
Huang, CY
Late-outgrowth endothelial progenitor cells (EPCs) are stress-resistant and responsible for reparative functions in the cardiovascular system. Oxidized-LDL (oxLDL) plays a critical role in cardiovascular disease pathogenesis. However, it is largely unknown what the impacts of oxLDL are on late-outgrowth EPCs. This study aimed to investigate the concentration-related effects of oxLDL on EPC functions and related angiogenesis, in vitro and in vivo. In this study, early and late-outgrowth EPCs were generated from circulating human mononuclear cells. oxLDL may regulate EPC vasculogenic function via the lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1). Lower concentrations (5 μg/mL) of oxLDL can potentiate EPC tube formation in vitro and in vivo by activating eNOS mechanisms, which are mediated by p38 MAPK- and SAPK/JNK-related pathways. Higher concentrations of oxLDL (10-50 μg/mL) impaired EPC function via the activation of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase pathways and consequent inhibition of eNOS activity, which could be reversed by anti-oxidants (diphenylene iodonium and apocynin) and gp91phox siRNA. In conclusion, oxLDL has concentration-dependent biphasic effects on human late-outgrowth EPC tube formation in vitro and in vivo.
Article
PLOS ONE.10(5):e0123971(2015)
journal article
PUBLIC LIBRARY SCIENCE
2015-05-27
application/pdf
PLOS ONE
5
10
e0123971
1932-6203
https://soar-ir.repo.nii.ac.jp/record/20084/files/25293063_08.pdf
eng
26017136
https://www.ncbi.nlm.nih.gov/pubmed/26017136
10.1371/journal.pone.0123971
https://doi.org/10.1371/journal.pone.0123971
© 2015 Lin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited