2024-03-29T02:05:42Z
https://soar-ir.repo.nii.ac.jp/oai
oai:soar-ir.repo.nii.ac.jp:02000344
2022-12-14T04:40:35Z
1016:1017
Myogenetic oligodeoxynucleotide complexed with berberine promotes differentiation of chicken myoblasts
Nihashi, Yuma
Shinji, Sayaka
Umezawa, Koji
Shimosato, Takeshi
Ono, Tamao
Kagami, Hiroshi
Takaya, Tomohide
Myoblasts are myogenic precursors that develop into myotubes during muscle formation. Improving efficiency of myoblast differentiation is important for advancing meat production by domestic animals. We recently identified novel oligodeoxynucleotides (ODNs) termed myogenetic ODNs (myoDNs) that promote the differentiation of mammalian myoblasts. An isoquinoline alkaloid, berberine, forms a complex with one of the myoDNs, iSN04, and enhances its activities. This study investigated the effects of myoDNs on chicken myoblasts to elucidate their species-specific actions. Seven myoDNs (iSN01-iSN07) were found to facilitate the differentiation of chicken myoblasts into myosin heavy chain (MHC)-positive myotubes. The iSN04-berberine complex exhibited a higher myogenetic activity than iSN04 alone, which was shown to enhance the differentiation of myoblasts into myotubes and the upregulated of myogenic gene expression (MyoD, myogenin, MHC, and myomaker). These data indicate that myoDNs promoting chicken myoblast differentiation may be used as potential feed additives in broiler diets.
Article
Animal Science Journal. 92(1) : e13597 (2021)
journal article
公益社団法人日本畜産学会
Wiley
2021-07-26
application/pdf
Animal Science Journal
1
92
e13597
e13597
1344-3941
AA11677214
https://soar-ir.repo.nii.ac.jp/record/2000344/files/AnimSciJ92.pdf
eng
10.1111/asj.13597