{"created":"2021-03-01T06:13:09.143171+00:00","id":10274,"links":{},"metadata":{"_buckets":{"deposit":"85668f77-9bae-49cd-a545-6fd8486ecf45"},"_deposit":{"id":"10274","owners":[],"pid":{"revision_id":0,"type":"depid","value":"10274"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00010274","sets":["1016:1017"]},"author_link":["32482"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"18","bibliographicPageEnd":"2396","bibliographicPageStart":"2385","bibliographicVolumeNumber":"318","bibliographic_titles":[{"bibliographic_title":"EXPERIMENTAL CELL RESEARCH"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Interleukin (IL)-3-dependent mouse bone marrow-derived mast cells (BMMCs) are an important model for studying the function of mucosal-type mast cells. In the present study, BMMCs were successfully immortalized by cell fusion using a hypoxanthine-aminopterin-thymidine medium-sensitive variant of P815 mouse mastocytoma (P815-6TgR) as a partner cell line. The established mouse mast cell hybridomas (MMCHs) expressed alpha, beta, and gamma subunits of high-affinity immunoglobulin E (IgE) receptor (FcERI) and possessed cytoplasmic granules devoid of or partially filled with electron-dense material. Four independent MMCH clones continuously proliferated without supplemental exogenous IL-3 and showed a degranulation response on stimulation with IgE+antigen. Furthermore, histamine synthesis and release by degranulation were confirmed in MMCH-D5, a MMCH clone that showed the strongest degranulation response. MMCH-D5 exhibited elevated levels of 1L-3, IL-4, IL-13, granulocyte-macrophage colony-stimulating factor, tumor necrosis factor (TNF)-alpha, and cyclooxygenase 2, and production of prostaglandin D2 and leukotriene C4 in response to IgE-induced stimulation. MMCH clones also expressed Toll-like receptors (TLRs) 1, 2, 4, and 6 and showed elevated levels of TNF-alpha expression in response to stimulation with TLR2 and TLR4 ligands. The MMCHs established using this method should be suitable for studies on FcERI- and TLR-mediated effector functions of mast cells.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"EXPERIMENTAL CELL RESEARCH. 318(18):2385-2396 (2012)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Kawahara, Takeshi","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.OVymPUkh.html"}]},"item_6_link_67":{"attribute_name":"WoS","attribute_value_mlt":[{"subitem_link_text":"Web of Science","subitem_link_url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000308259900011"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ELSEVIER INC"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"22824512"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubmed.ncbi.nlm.nih.gov/22824512/","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/j.yexcr.2012.07.010"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.yexcr.2012.07.010","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright© 2012 Elsevier Inc. All rights reserved."}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0014-4827","subitem_source_identifier_type":"ISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00641011","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kawahara,  Takeshi"}],"nameIdentifiers":[{"nameIdentifier":"32482","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-25"}],"displaytype":"detail","filename":"Establishment_characterization_mouse_bone_marrow-derived_mast_cell_hybridomas.pdf","filesize":[{"value":"3.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Establishment_characterization_mouse_bone_marrow-derived_mast_cell_hybridomas.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/10274/files/Establishment_characterization_mouse_bone_marrow-derived_mast_cell_hybridomas.pdf"},"version_id":"b765dd81-8a8f-4c52-abef-93b6ccce94b6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Mast cell","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell fusion","subitem_subject_scheme":"Other"},{"subitem_subject":"P815","subitem_subject_scheme":"Other"},{"subitem_subject":"Hybridoma","subitem_subject_scheme":"Other"},{"subitem_subject":"Degranulation","subitem_subject_scheme":"Other"},{"subitem_subject":"Toll-like receptor","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Establishment and characterization of mouse bone marrow-derived mast cell hybridomas","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Establishment and characterization of mouse bone marrow-derived mast cell hybridomas","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1017"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-08-27"},"publish_date":"2013-08-27","publish_status":"0","recid":"10274","relation_version_is_last":true,"title":["Establishment and characterization of mouse bone marrow-derived mast cell hybridomas"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:30:21.914515+00:00"}