@article{oai:soar-ir.repo.nii.ac.jp:00010351,
 author = {Egusa,  Shintaro and Otani,  Hajime},
 issue = {5},
 journal = {JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY},
 month = {Oct},
 note = {An immunostimulating glutamine-rich peptide was purified from a soybean protein fraction digested with Peptidase R produced by Rhizopus oryzae (Ro-digest) by a combination of SP-Sepharose column chromatography and reversed-phase high-performance liquid chromatography. The purified peptide was supposed to be located at or near the glutamine-rich region 202 to 222 of the glycinin G4 subunit. The peptide significantly increased the number of CD8(+), CD11b(+), and CD49b(+) cells in C3H/HeN mouse spleen cell cultures, while 2 chemically synthesized glutamine-rich peptides corresponding to residues 202 to 213 (QQQQQQKSHGGR) and residues 214 to 225 (KQGQHQQEEEEE) of the glycinin G4 subunit increased the number of interleukin (IL)-12(+)CD11b(+) cells. The peptide 202-213 also significantly increased the number of CD49b(+), IL-2(+)CD4(+), and interferon-gamma(+)CD4(+) cells and stimulated the cytotoxic activity of spleen cells toward the human erythroleukemia cell line K562. These results indicate that the glutamine-rich region of the soybean glycinin G4 subunit stimulates the cellular immune system in mouse spleen cell cultures., Article, JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY},
 pages = {428--433},
 title = {Characterization of a Cellular Immunostimulating Peptide from a Soybean Protein Fraction Digested with Peptidase R},
 volume = {55},
 year = {2009}
}