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Because mycelial colonies regenerated on minimum medium containing chloramphenicol only after the treatment of protoplast electrofusion, the regenerants were regarded as protoplast fusants. The fusants isolated from regenerated colonies were uninucleate, and their resistance of chloramphenicol seemed to be a stable trait. The fusants mated with P. pulmonarius but not with P. ostreatus, and showed almost identical random amplified polymorphic DNA (RAPD) patterns to that of the parental P. pulmonarius monokaryon. The sizes of mitochondrial genomes were estimated to be 81.5 kb for P. ostreatus monokaryon, 54.9 kb for P. pulmonarius monokaryon, and 84.5 or 86.0 kb for the fusants. BamHI and EcoRI digest patterns of the fusant mtDNAs were almost the same as the parental P. ostreatus monokaryon. From the above results, the fusants seemed to carry nuclei derived from the monokaryon of P. pulmonarius and mtDNA including the chloramphenicol-resistance gene from the P. ostreatus monokaryon, suggesting that the P. ostreatus mtDNA had been introduced into P. pulmonarius cells by interspecific protoplast fusion."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Fukuda, M"}], "nameIdentifiers": [{"nameIdentifier": "32975", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Wakayama, M"}], "nameIdentifiers": [{"nameIdentifier": "32976", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Uchida, M"}], "nameIdentifiers": [{"nameIdentifier": "32977", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fukumasa-Nakai, Y"}], "nameIdentifiers": [{"nameIdentifier": "32978", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsumoto, T"}], "nameIdentifiers": [{"nameIdentifier": "32979", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "mitochondrial_DNA_Pleurotus_ostreatus.pdf", "filesize": [{"value": "1.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 1100000.0, "url": {"label": "mitochondrial_DNA_Pleurotus_ostreatus.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/10424/files/mitochondrial_DNA_Pleurotus_ostreatus.pdf"}, "version_id": "9f357518-f758-46d6-b858-0d6a536e4306"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Introduction of mitochondrial DNA from Pleurotus ostreatus into Pleurotus pulmonarius by interspecific protoplast fusion", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Introduction of mitochondrial DNA from Pleurotus ostreatus into Pleurotus pulmonarius by interspecific protoplast fusion", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1017"], "permalink_uri": "http://hdl.handle.net/10091/489", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2007-11-14"}, "publish_date": "2007-11-14", "publish_status": "0", "recid": "10424", "relation": {}, "relation_version_is_last": true, "title": ["Introduction of mitochondrial DNA from Pleurotus ostreatus into Pleurotus pulmonarius by interspecific protoplast fusion"], "weko_shared_id": -1}
Introduction of mitochondrial DNA from Pleurotus ostreatus into Pleurotus pulmonarius by interspecific protoplast fusion
http://hdl.handle.net/10091/489
http://hdl.handle.net/10091/489b6f67ac0-6a6b-4aae-9423-fcdce257d5fc
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-11-14 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Introduction of mitochondrial DNA from Pleurotus ostreatus into Pleurotus pulmonarius by interspecific protoplast fusion | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Fukuda, M
× Fukuda, M× Wakayama, M× Uchida, M× Fukumasa-Nakai, Y× Matsumoto, T |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Fukuda, M | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.yCTNPpkh.html | |||||
出版者 | ||||||
出版者 | Springer Japan | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Wood Science. 53(4):339-343 (2007) | |||||
書誌情報 |
Journal of Wood Science 巻 53, 号 4, p. 339-343, 発行日 2007-08 |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The original publication is available at www.springerllink.com. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1435-0211 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11200007 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1007/s10086-006-0861-9 | |||||
関連名称 | 10.1007/s10086-006-0861-9 | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000248814900011 |