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Following the creation of a new L-lysine producer Corynebacterium glutamicum AHP-3 that carried three useful mutations (lysC311, hom59, and pyc458) on the relevant downstream pathways, we shifted our target to the pentose phosphate pathway. Comparative genomic analysis for the pathway between a classically derived L-lysine producer and its parental wild-type identified several mutations. Among these mutations, a Ser-361 --\u003e Phe mutation in the 6-phosphogluconate dehydrogenase gene (gnd) was defined as a useful mutation for L-lysine production. Introduction of the gnd mutation into strain AHP-3 by allelic replacement led to approximately 15% increased L-lysine production. Enzymatic analysis revealed that the mutant enzyme was less sensitive than the wild-type enzyme,to allosteric inhibition by intracellular metabolites, such as fructose 1,6-bisphosphate, D-glyceraldehyde 3-phosphate, phosphoribosyl pyrophosphate, ATP, and NADPH, which were known to inhibit this enzyme. 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  1. 070 農学部, 大学院農学研究科
  2. 0701 学術論文

A novel gnd mutation leading to increased L-lysine production in Corynebacterium glutamicum

http://hdl.handle.net/10091/200
http://hdl.handle.net/10091/200
5b107a9e-b122-459f-b499-1b3510297d5a
名前 / ファイル ライセンス アクション
A A novel gnd mutation.pdf (279.4 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2007-05-07
タイトル
言語 en
タイトル A novel gnd mutation leading to increased L-lysine production in Corynebacterium glutamicum
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Ohnishi, J

× Ohnishi, J

WEKO 33017

Ohnishi, J

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Katahira, R

× Katahira, R

WEKO 33018

Katahira, R

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Mitsuhashi, S

× Mitsuhashi, S

WEKO 33019

Mitsuhashi, S

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Kakita, S

× Kakita, S

WEKO 33020

Kakita, S

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Ikeda, M

× Ikeda, M

WEKO 33021

Ikeda, M

Search repository
信州大学研究者総覧へのリンク
氏名 Ikeda, M
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html
出版者
出版者 ELSEVIER SCIENCE BV
引用
内容記述タイプ Other
内容記述 Fems Microbiology Letters. 242(2): 265-274 (2005)
書誌情報 FEMS microbiology letters

巻 242, 号 2, p. 265-274, 発行日 2005-01-15
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0378-1097
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00193861
他の資源との関係:URI
識別子タイプ URI
関連識別子 http://www.elsevier.com/wps/find/journaldescription.cws_home/506058/description
関連名称 http://www.elsevier.com/wps/find/journaldescription.cws_home/506058/description
PubMed
識別子タイプ PMID
関連識別子 https://pubmed.ncbi.nlm.nih.gov/15621447/
関連名称 15621447
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.femsle.2004.11.014
関連名称 10.1016/j.femsle.2004.11.014
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000226405900011
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