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  1. 070 農学部, 大学院農学研究科
  2. 0702 紀要 ・刊行物
  3. 07021 信州大学農学部紀要
  4. Vol. 33

The Effect of EDTA on Incorporation of [3H] Methionine into Two-Cell Mouse Embryos

http://hdl.handle.net/10091/899
c55aea55-cb51-4940-9d2c-f3e3a6ab5748
名前 / ファイル ライセンス アクション
v33_02.pdf v33_02.pdf (289.3 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2008-01-16
タイトル
タイトル The Effect of EDTA on Incorporation of [3H] Methionine into Two-Cell Mouse Embryos
言語
言語 eng
キーワード
主題Scheme Other
主題 two-cell block
キーワード
主題Scheme Other
主題 incorporation of methionine
キーワード
主題Scheme Other
主題 EDTA
キーワード
主題Scheme Other
主題 mouse
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
その他(別言語等)のタイトル
その他のタイトル マウス2細胞卵子における3H-メチオニンの取込みに及ぼすEDTAの影響
著者 Tsujii, H

× Tsujii, H

WEKO 33469

Tsujii, H

Search repository
Sakai, H

× Sakai, H

WEKO 33470

Sakai, H

Search repository
Takagi, Y

× Takagi, Y

WEKO 33471

Takagi, Y

Search repository
信州大学研究者総覧へのリンク
氏名 Takagi, Y
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.yNTUPpkh.html
出版者
出版者 信州大学農学部
引用
内容記述タイプ Other
内容記述 信州大学農学部紀要. 33(1-2): 9-17 (1996)
書誌情報 信州大学農学部紀要

巻 33, 号 1-2, p. 9-17, 発行日 1996-12-31
抄録
内容記述タイプ Abstract
内容記述 To investigate the effect of EDTA on the in vitro development of mouse embryos from 1-cell to blastocyst, the embryos were cultured in M 16 and M 16 plus EDTA. The incorporation of[3H]methionine was also compared. A significant increase in the number of four-cell embryos (87.5 %) and blastocysts (71.9 %) was observed when embryos were cultured in M16 with EDTA compared to basic M16 medium (23.5 % and 2.9 %, respectively). When considering the results of first two incorporation experiments together, the lowest incorporation of[3H]methionine was observed at 45 h after hCG injection in both medium. So, we may explain that cell block in the in vitro embryo development occurred within a relatively narrow range of time, during 40-45 h after hCG injection. On the other hand the incorporation of[3H]methionine differed between ICR and C57B/6 strains and indicated that in blocking strain mice care must be taken to ensure that embryos at the 2-cell stage are recovered from the oviducts late enough in the second cell cycle for their normal development.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0583-0621
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00121352
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