{"_buckets": {"deposit": "f7ddc166-0cf7-48e1-ae46-62f7231d1a88"}, "_deposit": {"id": "10589", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "10589"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00010589"}, "item_6_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\uff08\u5225\u8a00\u8a9e\u7b49\uff09\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\u30de\u30a6\u30b9\uff12\u7d30\u80de\u5375\u5b50\u306b\u304a\u3051\u308b3H-\u30e1\u30c1\u30aa\u30cb\u30f3\u306e\u53d6\u8fbc\u307f\u306b\u53ca\u307c\u3059EDTA\u306e\u5f71\u97ff"}]}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1996-12-31", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1-2", "bibliographicPageEnd": "17", "bibliographicPageStart": "9", "bibliographicVolumeNumber": "33", "bibliographic_titles": [{"bibliographic_title": "\u4fe1\u5dde\u5927\u5b66\u8fb2\u5b66\u90e8\u7d00\u8981"}]}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "To investigate the effect of EDTA on the in vitro development of mouse embryos from 1-cell to blastocyst, the embryos were cultured in M 16 and M 16 plus EDTA. The incorporation of\uff3b3H\uff3dmethionine was also compared. A significant increase in the number of four-cell embryos (87.5 %) and blastocysts (71.9 %) was observed when embryos were cultured in M16 with EDTA compared to basic M16 medium (23.5 % and 2.9 %, respectively). When considering the results of first two incorporation experiments together, the lowest incorporation of\uff3b3H\uff3dmethionine was observed at 45 h after hCG injection in both medium. So, we may explain that cell block in the in vitro embryo development occurred within a relatively narrow range of time, during 40-45 h after hCG injection. On the other hand the incorporation of\uff3b3H\uff3dmethionine differed between ICR and C57B/6 strains and indicated that in blocking strain mice care must be taken to ensure that embryos at the 2-cell stage are recovered from the oviducts late enough in the second cell cycle for their normal development.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_31": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\uff1amime\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "\u4fe1\u5dde\u5927\u5b66\u8fb2\u5b66\u90e8\u7d00\u8981. 33(1-2): 9-17 (1996)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Takagi, Y", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.yNTUPpkh.html"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u4fe1\u5dde\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0583-0621", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0583-0621", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00121352", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_66": {"attribute_name": "sortkey", "attribute_value_mlt": [{"subitem_text_value": "02"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Tsujii,  H"}], "nameIdentifiers": [{"nameIdentifier": "33469", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakai,  H"}], "nameIdentifiers": [{"nameIdentifier": "33470", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takagi,  Y"}], "nameIdentifiers": [{"nameIdentifier": "33471", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "v33_02.pdf", "filesize": [{"value": "289.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 289300.0, "url": {"label": "v33_02.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/10589/files/v33_02.pdf"}, "version_id": "707916a1-de33-47a3-a957-6d7d3eb13ff0"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "two-cell block", "subitem_subject_scheme": "Other"}, {"subitem_subject": "incorporation of methionine", "subitem_subject_scheme": "Other"}, {"subitem_subject": "EDTA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "mouse", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "The Effect of EDTA on Incorporation\u3000of\u3000[3H\uff3d Methionine into Two-Cell Mouse Embryos", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "The Effect of EDTA on Incorporation\u3000of\u3000[3H\uff3d Methionine into Two-Cell Mouse Embryos"}]}, "item_type_id": "6", "owner": "1", "path": ["1016/1018/1019/1038"], "permalink_uri": "http://hdl.handle.net/10091/899", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2008-01-16"}, "publish_date": "2008-01-16", "publish_status": "0", "recid": "10589", "relation": {}, "relation_version_is_last": true, "title": ["The Effect of EDTA on Incorporation\u3000of\u3000[3H\uff3d Methionine into Two-Cell Mouse Embryos"], "weko_shared_id": null}