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The Effect of EDTA on Incorporation of [3H] Methionine into Two-Cell Mouse Embryos
http://hdl.handle.net/10091/899
http://hdl.handle.net/10091/899c55aea55-cb51-4940-9d2c-f3e3a6ab5748
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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公開日 | 2008-01-16 | |||||||||||
タイトル | ||||||||||||
タイトル | The Effect of EDTA on Incorporation of [3H] Methionine into Two-Cell Mouse Embryos | |||||||||||
言語 | ||||||||||||
言語 | eng | |||||||||||
キーワード | ||||||||||||
主題 | two-cell block, incorporation of methionine, EDTA, mouse | |||||||||||
資源タイプ | ||||||||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
タイプ | journal article | |||||||||||
その他(別言語等)のタイトル | ||||||||||||
その他のタイトル | マウス2細胞卵子における3H-メチオニンの取込みに及ぼすEDTAの影響 | |||||||||||
言語 | ja | |||||||||||
著者 |
Tsujii, H
× Tsujii, H
× Sakai, H
× Takagi, Y
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信州大学研究者総覧へのリンク | ||||||||||||
氏名 | Takagi, Y | |||||||||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.yNTUPpkh.html | |||||||||||
出版者 | ||||||||||||
出版者 | 信州大学農学部 | |||||||||||
引用 | ||||||||||||
内容記述 | 信州大学農学部紀要. 33(1-2): 9-17 (1996) | |||||||||||
書誌情報 |
信州大学農学部紀要 巻 33, 号 1-2, p. 9-17, 発行日 1996-12-31 |
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抄録 | ||||||||||||
内容記述 | To investigate the effect of EDTA on the in vitro development of mouse embryos from 1-cell to blastocyst, the embryos were cultured in M 16 and M 16 plus EDTA. The incorporation of[3H]methionine was also compared. A significant increase in the number of four-cell embryos (87.5 %) and blastocysts (71.9 %) was observed when embryos were cultured in M16 with EDTA compared to basic M16 medium (23.5 % and 2.9 %, respectively). When considering the results of first two incorporation experiments together, the lowest incorporation of[3H]methionine was observed at 45 h after hCG injection in both medium. So, we may explain that cell block in the in vitro embryo development occurred within a relatively narrow range of time, during 40-45 h after hCG injection. On the other hand the incorporation of[3H]methionine differed between ICR and C57B/6 strains and indicated that in blocking strain mice care must be taken to ensure that embryos at the 2-cell stage are recovered from the oviducts late enough in the second cell cycle for their normal development. | |||||||||||
資源タイプ(コンテンツの種類) | ||||||||||||
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収録物識別子タイプ | ISSN | |||||||||||
収録物識別子 | 0583-0621 | |||||||||||
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収録物識別子タイプ | NCID | |||||||||||
収録物識別子 | AN00121352 | |||||||||||
出版タイプ | ||||||||||||
出版タイプ | VoR | |||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Cite as
Tsujii, H, Sakai, H, Takagi, Y, 1996, The Effect of EDTA on Incorporation of [3H] Methionine into Two-Cell Mouse Embryos: 信州大学農学部, 9–17 p.
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