{"created":"2021-03-01T06:13:28.256777+00:00","id":10589,"links":{},"metadata":{"_buckets":{"deposit":"f7ddc166-0cf7-48e1-ae46-62f7231d1a88"},"_deposit":{"id":"10589","owners":[],"pid":{"revision_id":0,"type":"depid","value":"10589"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00010589","sets":["1016:1018:1019:1038"]},"author_link":["33469","33470","33471"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"マウス２細胞卵子における3H-メチオニンの取込みに及ぼすEDTAの影響","subitem_alternative_title_language":"ja"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-12-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-2","bibliographicPageEnd":"17","bibliographicPageStart":"9","bibliographicVolumeNumber":"33","bibliographic_titles":[{"bibliographic_title":"信州大学農学部紀要"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To investigate the effect of EDTA on the in vitro development of mouse embryos from 1-cell to blastocyst, the embryos were cultured in M 16 and M 16 plus EDTA. The incorporation of［3H］methionine was also compared. A significant increase in the number of four-cell embryos (87.5 %) and blastocysts (71.9 %) was observed when embryos were cultured in M16 with EDTA compared to basic M16 medium (23.5 % and 2.9 %, respectively). When considering the results of first two incorporation experiments together, the lowest incorporation of［3H］methionine was observed at 45 h after hCG injection in both medium. So, we may explain that cell block in the in vitro embryo development occurred within a relatively narrow range of time, during 40-45 h after hCG injection. On the other hand the incorporation of［3H］methionine differed between ICR and C57B/6 strains and indicated that in blocking strain mice care must be taken to ensure that embryos at the 2-cell stage are recovered from the oviducts late enough in the second cell cycle for their normal development.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"信州大学農学部紀要. 33(1-2): 9-17 (1996)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Takagi, Y","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.yNTUPpkh.html"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"信州大学農学部"}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0583-0621","subitem_source_identifier_type":"ISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00121352","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tsujii,  H"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakai,  H"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takagi,  Y"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-25"}],"displaytype":"detail","filename":"v33_02.pdf","filesize":[{"value":"289.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"v33_02.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/10589/files/v33_02.pdf"},"version_id":"707916a1-de33-47a3-a957-6d7d3eb13ff0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"two-cell block","subitem_subject_scheme":"Other"},{"subitem_subject":"incorporation of methionine","subitem_subject_scheme":"Other"},{"subitem_subject":"EDTA","subitem_subject_scheme":"Other"},{"subitem_subject":"mouse","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The Effect of EDTA on Incorporation　of　[3H］ Methionine into Two-Cell Mouse Embryos","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Effect of EDTA on Incorporation　of　[3H］ Methionine into Two-Cell Mouse Embryos","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1038"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-01-16"},"publish_date":"2008-01-16","publish_status":"0","recid":"10589","relation_version_is_last":true,"title":["The Effect of EDTA on Incorporation　of　[3H］ Methionine into Two-Cell Mouse Embryos"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-06T02:53:52.171327+00:00"}