{"created":"2021-03-01T06:13:28.831831+00:00","id":10598,"links":{},"metadata":{"_buckets":{"deposit":"7a10eedd-2577-4709-a436-d298483b3b5b"},"_deposit":{"id":"10598","owners":[],"pid":{"revision_id":0,"type":"depid","value":"10598"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00010598","sets":["1016:1018:1019:1039"]},"author_link":["33491","33492","33493"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_6_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ペプチド合成またはＺ(ベンジルオキシカルボニル） : アミノ酸エステル加水分解における２，３のプロテアーゼの微環境へのｎ-アルコール効果の多様性","subitem_alternative_title_language":"ja"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-12-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-2","bibliographicPageEnd":"22","bibliographicPageStart":"15","bibliographicVolumeNumber":"32","bibliographic_titles":[{"bibliographic_title":"信州大学農学部紀要"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To investigate the interaction of proteases (thermolysin, α-chymotrypsin, papain and pepsin) with organic solvent molecules, buffer systems in which n-alcohols were dissolved as to make a one-phase medium were used in the case of peptide synthesis or Z-amino acid hydrolysis as model reactions. The chain length of n-alcohols gave different characteristics of the effective patterns to the enzymes, and also each protease was affected in different modes of effective profiles by n-alcohols. Activity of α-chymotrypsin was increased by n-alcohols higher than n-hexanol grdually with increase of their concentration just over the saturation to the buffer. The activation was proposed to be non-essential by kinetics. Meanwhile, n-pentanol to α-chymotrypsin showed to be an uncompetitive inhibitor.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"信州大学農学部紀要. 32(1-2): 15-22 (1995)","subitem_description_type":"Other"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"信州大学農学部"}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0583-0621","subitem_source_identifier_type":"ISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00121352","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sato,  K"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tadasa,  K"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kayahara,  H"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-25"}],"displaytype":"detail","filename":"v32_03.pdf","filesize":[{"value":"345.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"v32_03.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/10598/files/v32_03.pdf"},"version_id":"de454218-236c-4e60-99b5-dc7e5cbf405b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"protease","subitem_subject_scheme":"Other"},{"subitem_subject":"microenvironment","subitem_subject_scheme":"Other"},{"subitem_subject":"thermolysin","subitem_subject_scheme":"Other"},{"subitem_subject":"α-chymotrypsin","subitem_subject_scheme":"Other"},{"subitem_subject":"papain","subitem_subject_scheme":"Other"},{"subitem_subject":"pepsin","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Variety of Effect of n-Alcohols on Microenvironment of Some Proteases in Peptide Synthesis or Z (N-Benzyloxycarbonyl) : Amino Acid Ester Hydrolysis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Variety of Effect of n-Alcohols on Microenvironment of Some Proteases in Peptide Synthesis or Z (N-Benzyloxycarbonyl) : Amino Acid Ester Hydrolysis","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1039"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-12-28"},"publish_date":"2007-12-28","publish_status":"0","recid":"10598","relation_version_is_last":true,"title":["Variety of Effect of n-Alcohols on Microenvironment of Some Proteases in Peptide Synthesis or Z (N-Benzyloxycarbonyl) : Amino Acid Ester Hydrolysis"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-06T02:52:09.540309+00:00"}