{"created":"2021-03-01T06:13:29.824310+00:00","id":10614,"links":{},"metadata":{"_buckets":{"deposit":"e84c2c61-80c6-457d-a1c2-63c0f742fd51"},"_deposit":{"id":"10614","owners":[],"pid":{"revision_id":0,"type":"depid","value":"10614"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00010614","sets":["1016:1018:1019:1040"]},"author_link":["33534","33535","33536","33537","33538"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-12-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"144","bibliographicPageStart":"139","bibliographicVolumeNumber":"31","bibliographic_titles":[{"bibliographic_title":"信州大学農学部紀要"}]}]},"item_10_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"木曽馬13頭の血液よりミトコンドリアDNA(mtDNA)を精製し,D-loop領域をPCR増幅した後,制限酵素処理することにより多型性の検出を試みた(PCR-RFLP)。すなわち,3種類の制限酵素AciI,Cfr13IおよびMseIによるRFLPパターンをポリアクリルアミドゲル電気泳動により検出し,その出現頻度をサラブレッド種とその他の品種で報告されている結果と比較した。 AciIとMseIでは,それぞれ2つのタイプのRFLPが見出され,このうちMseIにおけるC型はこれまで他の品種では認められていない新しいRFLPパターンであった。Cfr13Iにおいては多型は認められなかった。これらのRFLPパターンの出現頻度は,AciIでA型.0.769,C型.0.231,MseIでB型.0.692,C型.0.308であった。 木曽馬のmtDNA D-loop領域のPCR-RFLP解析の結果は,全体的にサラブレッドよりもそれ以外の品種における結果とより類似した傾向を示した。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10_description_30":{"attribute_name":"資源タイプ(コンテンツの種類)","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_10_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"信州大学農学部紀要. 31(2): 139-144 (1994)","subitem_description_type":"Other"}]},"item_10_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"信州大学農学部"}]},"item_10_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0583-0621","subitem_source_identifier_type":"ISSN"}]},"item_10_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00121352","subitem_source_identifier_type":"NCID"}]},"item_1627890569677":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"廣村, 恵子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"石田, 信繁"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"坂上, 正行"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"向山, 明孝"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"辻井, 弘忠"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-25"}],"displaytype":"detail","filename":"v31n02_08.pdf","filesize":[{"value":"502.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"v31n02_08.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/10614/files/v31n02_08.pdf"},"version_id":"26b2d8f5-54a3-4453-a4d1-e9c44425af1c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"木曽馬","subitem_subject_scheme":"Other"},{"subitem_subject":"mtDNA","subitem_subject_scheme":"Other"},{"subitem_subject":"PCR-RFLP","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"木曽馬におけるミトコンドリアDNA D-loop領域のPCR-RFLP解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"木曽馬におけるミトコンドリアDNA D-loop領域のPCR-RFLP解析","subitem_title_language":"ja"},{"subitem_title":"PCR-RFLP Analysis of Mitochondorial DNA D-loop Region in Kiso Native Horses","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["1040"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-12-28"},"publish_date":"2007-12-28","publish_status":"0","recid":"10614","relation_version_is_last":true,"title":["木曽馬におけるミトコンドリアDNA D-loop領域のPCR-RFLP解析"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-06T02:08:58.721558+00:00"}