@article{oai:soar-ir.repo.nii.ac.jp:00010731, author = {唐澤, 豊 and 高瀬, 能久 and 木部, 久衛}, issue = {1}, journal = {信州大学農学部紀要}, month = {Jul}, note = {This experiment was carried out to examine the effect of pH value or lactic acid content of silage extract medium on metabolites by clostridia and on quality of the culture as silage. Initial pH values of the silage extract media consisted of silage juice plus pulp cellulose powder were adjusted to 4.1, 4.9 and 5.8, and lactic acid contents of the media were to 2.5, 5.0, 6.7, 13.0 and 15.0%. Clostridia tested in this experiment were Cl. scatologenes, Cl. butyricum, Cl. tyrobutyricum and Cl. subterminale isolated from corn silage. In silage extract media with initial pH 4, butyric acid was not produced, pH values and lactic acid contents were not changed and ammonia contents were little increased by Cl. scatologenes, Cl. tyrobutyricum or Cl. subterminale. Little or no increase in butyric acid and ammonia contents and in pH value in the silage extract medium inoculated with Clm butyricum was observed even when the initial pH was 5. No production of butyric acid, little change in pH value and less reduction of lactic acid were caused by Cl. scatologenes and Cl. tyrobutyricum when the initial lactic acid content was 13%, by Cl. subterminale when it was 15% and by Cl. butyicum when it was 6.7%. The estimation of the silage extract cultures by Flieg's method showed the reduction of the silage quality to be inhibited by the initial medium pH of 4 and by the initial lactic acid content of 13.0%. These results suggest that the reduction of the silage quality by clostridia is inhibited when the silage is lower than 4 in pH value or contains lactic acid of more than 13%., Article, 信州大学農学部紀要 20(1): 117-126(1983)}, pages = {117--126}, title = {サイレージより分離した嫌気性芽胞菌の代謝に及ぼす培地のpHと乳酸含量の影響}, volume = {20}, year = {1983} }