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Activity of the purified enzyme was progrssively inhibited with increasing of p-chloromercuribenzoate and iodoacetate, but not by o-phenanthroline and diisopropylfluorophosphate. This fact strongly suggest that a free sulfhydyl group(s) is required for activity. The possible involvement of this functional group, then, determined by treating the enzyme with iodine and/or glutathione. As results, activity of the enzyme was reactivated considerably by glutathione, and addition of glutathione to iodine oxidized enzyme resulted in 73.7% reactivity, whereas iodine oxidation of the enzyme resulted in 52.6% reduction in activity.", "subitem_description_type": "Abstract"}]}, "item_10_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_10_description_31": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\uff1amime\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_10_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "\u4fe1\u5dde\u5927\u5b66\u8fb2\u5b66\u90e8\u7d00\u8981 13(1): 13-20(1976)", "subitem_description_type": "Other"}]}, "item_10_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u4fe1\u5dde\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_10_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0583-0621", "subitem_source_identifier_type": "ISSN"}]}, "item_10_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0583-0621", "subitem_source_identifier_type": "ISSN"}]}, "item_10_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00121352", "subitem_source_identifier_type": "NCID"}]}, "item_10_text_66": {"attribute_name": "sortkey", "attribute_value_mlt": [{"subitem_text_value": "02"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "HOSONO,  Akiyoshi"}], "nameIdentifiers": [{"nameIdentifier": "34005", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TOKITA,  Fumisaburo"}], "nameIdentifiers": [{"nameIdentifier": "34006", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Agriculture13-01-02.pdf", "filesize": [{"value": "357.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 357700.0, "url": {"label": "Agriculture13-01-02.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/10810/files/Agriculture13-01-02.pdf"}, "version_id": "6402de13-5ffc-4e44-9878-0d3701f28e6e"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Inhibitor and activator requirements of the intracellular protease of Leuconostoc citrovorum", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Inhibitor and activator requirements of the intracellular protease of Leuconostoc citrovorum"}]}, "item_type_id": "10", "owner": "1", "path": ["1016/1018/1019/1058"], "permalink_uri": "http://hdl.handle.net/10091/2303", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2009-03-03"}, "publish_date": "2009-03-03", "publish_status": "0", "recid": "10810", "relation": {}, "relation_version_is_last": true, "title": ["Inhibitor and activator requirements of the intracellular protease of Leuconostoc citrovorum"], "weko_shared_id": null}