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Inhibitor and activator requirements of the intracellular protease of Leuconostoc citrovorum
http://hdl.handle.net/10091/2303
http://hdl.handle.net/10091/2303f8e3b620-5e1d-4702-b86b-099cfd37e700
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Agriculture13-01-02.pdf (357.7 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2009-03-03 | |||||||||
| タイトル | ||||||||||
| タイトル | Inhibitor and activator requirements of the intracellular protease of Leuconostoc citrovorum | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| タイプ | departmental bulletin paper | |||||||||
| その他（別言語等）のタイトル | ||||||||||
| その他のタイトル | Leuconostoc citrovorumの菌体内プロテアーゼの活性阻害剤ならびに賦活剤 | |||||||||
| 言語 | ja | |||||||||
| 著者 |
HOSONO, Akiyoshi
× HOSONO, Akiyoshi
× TOKITA, Fumisaburo
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| 出版者 | ||||||||||
| 出版者 | 信州大学農学部 | |||||||||
| 引用 | ||||||||||
| 内容記述 | 信州大学農学部紀要 13(1): 13-20(1976) | |||||||||
| 書誌情報 |
信州大学農学部紀要 巻 13, 号 1, p. 13-20, 発行日 1976-06-25 |
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| 抄録 | ||||||||||
| 内容記述 | In the present study, the intracellular protease of Leuconostoc citrovorum was purified by ammonium precipitation and by Sephadex column and DEAE-cellulose column chromatography. Activity of the purified enzyme was progrssively inhibited with increasing of p-chloromercuribenzoate and iodoacetate, but not by o-phenanthroline and diisopropylfluorophosphate. This fact strongly suggest that a free sulfhydyl group(s) is required for activity. The possible involvement of this functional group, then, determined by treating the enzyme with iodine and/or glutathione. As results, activity of the enzyme was reactivated considerably by glutathione, and addition of glutathione to iodine oxidized enzyme resulted in 73.7% reactivity, whereas iodine oxidation of the enzyme resulted in 52.6% reduction in activity. | |||||||||
| 資源タイプ（コンテンツの種類） | ||||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 0583-0621 | |||||||||
| 書誌レコードID | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AN00121352 | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
