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Inhibitor and activator requirements of the intracellular protease of Leuconostoc citrovorum
http://hdl.handle.net/10091/2303
http://hdl.handle.net/10091/2303f8e3b620-5e1d-4702-b86b-099cfd37e700
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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公開日 | 2009-03-03 | |||||||||
タイトル | ||||||||||
タイトル | Inhibitor and activator requirements of the intracellular protease of Leuconostoc citrovorum | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
資源タイプ | ||||||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||
タイプ | departmental bulletin paper | |||||||||
その他(別言語等)のタイトル | ||||||||||
その他のタイトル | Leuconostoc citrovorumの菌体内プロテアーゼの活性阻害剤ならびに賦活剤 | |||||||||
言語 | ja | |||||||||
著者 |
HOSONO, Akiyoshi
× HOSONO, Akiyoshi
× TOKITA, Fumisaburo
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出版者 | ||||||||||
出版者 | 信州大学農学部 | |||||||||
引用 | ||||||||||
内容記述 | 信州大学農学部紀要 13(1): 13-20(1976) | |||||||||
書誌情報 |
信州大学農学部紀要 巻 13, 号 1, p. 13-20, 発行日 1976-06-25 |
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抄録 | ||||||||||
内容記述 | In the present study, the intracellular protease of Leuconostoc citrovorum was purified by ammonium precipitation and by Sephadex column and DEAE-cellulose column chromatography. Activity of the purified enzyme was progrssively inhibited with increasing of p-chloromercuribenzoate and iodoacetate, but not by o-phenanthroline and diisopropylfluorophosphate. This fact strongly suggest that a free sulfhydyl group(s) is required for activity. The possible involvement of this functional group, then, determined by treating the enzyme with iodine and/or glutathione. As results, activity of the enzyme was reactivated considerably by glutathione, and addition of glutathione to iodine oxidized enzyme resulted in 73.7% reactivity, whereas iodine oxidation of the enzyme resulted in 52.6% reduction in activity. | |||||||||
資源タイプ(コンテンツの種類) | ||||||||||
ISSN | ||||||||||
収録物識別子タイプ | ISSN | |||||||||
収録物識別子 | 0583-0621 | |||||||||
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収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AN00121352 | |||||||||
出版タイプ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |