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  1. 070 農学部, 大学院農学研究科
  2. 0702 紀要 ・刊行物
  3. 07021 信州大学農学部紀要
  4. Vol. 13

Inhibitor and activator requirements of the intracellular protease of Leuconostoc citrovorum

http://hdl.handle.net/10091/2303
http://hdl.handle.net/10091/2303
f8e3b620-5e1d-4702-b86b-099cfd37e700
名前 / ファイル ライセンス アクション
Agriculture13-01-02.pdf Agriculture13-01-02.pdf (357.7 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2009-03-03
タイトル
言語 en
タイトル Inhibitor and activator requirements of the intracellular protease of Leuconostoc citrovorum
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
その他(別言語等)のタイトル
その他のタイトル Leuconostoc citrovorumの菌体内プロテアーゼの活性阻害剤ならびに賦活剤
言語 ja
著者 HOSONO, Akiyoshi

× HOSONO, Akiyoshi

WEKO 34005

HOSONO, Akiyoshi

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TOKITA, Fumisaburo

× TOKITA, Fumisaburo

WEKO 34006

TOKITA, Fumisaburo

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出版者
出版者 信州大学農学部
引用
内容記述タイプ Other
内容記述 信州大学農学部紀要 13(1): 13-20(1976)
書誌情報 信州大学農学部紀要

巻 13, 号 1, p. 13-20, 発行日 1976-06-25
抄録
内容記述タイプ Abstract
内容記述 In the present study, the intracellular protease of Leuconostoc citrovorum was purified by ammonium precipitation and by Sephadex column and DEAE-cellulose column chromatography. Activity of the purified enzyme was progrssively inhibited with increasing of p-chloromercuribenzoate and iodoacetate, but not by o-phenanthroline and diisopropylfluorophosphate. This fact strongly suggest that a free sulfhydyl group(s) is required for activity. The possible involvement of this functional group, then, determined by treating the enzyme with iodine and/or glutathione. As results, activity of the enzyme was reactivated considerably by glutathione, and addition of glutathione to iodine oxidized enzyme resulted in 73.7% reactivity, whereas iodine oxidation of the enzyme resulted in 52.6% reduction in activity.
言語 en
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0583-0621
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00121352
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
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