@article{oai:soar-ir.repo.nii.ac.jp:00012266,
 author = {KOENUMA,  AKIRA},
 issue = {2},
 journal = {信州大学理学部紀要},
 month = {Mar},
 note = {On the insemination of the sea urchin eggs, Hemicentrotus pulcherrimus, the effects of the modified spermatozoa were investigated. In the cases where the eggs were inseminated with the spermatozoa treated with the alkaline sea water, pH 9.2, abnormally cleavaged eggs increased. In these abnormal eggs there seems to be a pretty number of the polyspermic eggs. From the insemination with the low-Caz z -treated spermatozoa a somewhat increase of the abnormal eggs resulted. In the cases of the insemination with the spermatozoa treated with uranyl nitrate solution an increase of the abnormal eggs did not result from the first insemination with the modified sperm, but from the second insemination. These results show a possibility that those treatments of the spermatozoa affect in an early process of fertilization. As to the results of these experiments, effects of treatments of the spermatozoa were discussed with a relation to the acrosome reaction of the spermatozoa., Article, 信州大学理学部紀要 4(2): 71-78(1970)},
 pages = {71--78},
 title = {Effects of the treatments of the spermatozoa with alkaline sea water, low-Caz z  solution and uranyl nitrate solution on the insemination of sea urchin eggs, Hemicentrotus pulcherrimus.},
 volume = {4},
 year = {1970}
}