@article{oai:soar-ir.repo.nii.ac.jp:00012298,
 author = {KOENUMA,  AKIRA},
 issue = {1},
 journal = {信州大学理学部紀要},
 month = {Mar},
 note = {Philosama cynthia cynthia is one of those insects whose pupal diapause is determined by the endocrine activity of the neurosecretory cells of the brain. Two substances were extracted from the brain of Bombyx mori independently, and they were identified with the brain hormone of insects concerned with their metamorphosis. One of them was reported as cholesterol. This paper presents the result of the experiments carried out to examine the hormonal action of cholesterol on the development of the diapausing pupa in Philosamia cynthia cynthia. In one group of the experiments, various doses of cholesterol suspended in 0.02ml of 9% ethyl alcohol were injected into the fourth or fifth abdominal segment of the diapausing pupa, then they were incubated at 25℃ for about 250 days. Cholesterol by Nutritional Biochemical Co. (N. B. C.) U. S. A. and by Tokyo Kasei Kogyo Co. Tokyo was used. In the other group of experiments, cholesterol was injected to the decerebrated pupa. Any evidence suggesting the hormonal action on the development of the diapausing pupa was not obtained from the experiments in which cholesterol was injected to the pupa, since none out of the 20 pupae developed to imago in all cases when they were incubated at 25℃ for about 250 days. Therefore, as far as present experiments were concerned, it seems to be improbable that pure cholesterol has some effect on the pupal diapause in Philosamia cynthia cynthia., Article, 信州大学理学部紀要 1(1): 55-63(1967)},
 pages = {55--63},
 title = {The Effect of Cholesterol on the Adult Development of Diapausing Pupae of the Giant Silkmoth, Philosamia cynthia cynthia},
 volume = {1},
 year = {1967}
}