@article{oai:soar-ir.repo.nii.ac.jp:00013354, author = {Matsumura, Hideo and Miyagi, Norimichi and Taniai, Naoki and Fukushima, Mai and Tarora, Kazuhiko and Shudo, Ayano and Urasaki, Naoya}, issue = {1}, journal = {PLOS ONE}, month = {Jan}, note = {Momordica charantia is a monoecious plant of the Cucurbitaceae family that has both male and female unisexual flowers. Its unique gynoecious line, OHB61-5, is essential as a maternal parent in the production of F-1 cultivars. To identify the DNA markers for this gynoecy, a RAD-seq (restriction-associated DNA tag sequencing) analysis was employed to reveal genome-wide DNA polymorphisms and to genotype the F-2 progeny from a cross between OHB61-5 and a monoecious line. Based on a RAD-seq analysis of F-2 individuals, a linkage map was constructed using 552 co-dominant markers. In addition, after analyzing the pooled genomic DNA from monoecious or gynoecious F-2 plants, several SNP loci that are genetically linked to gynoecy were identified. GTFL-1, the closest SNP locus to the putative gynoecious locus, was converted to a conventional DNA marker using invader assay technology, which is applicable to the marker-assisted selection of gynoecy in M. charantia breeding., Article, PLoS One 9(1):e87138 (2014)}, title = {Mapping of the Gynoecy in Bitter Gourd (Momordica charantia) Using RAD-Seq Analysis}, volume = {9}, year = {2014} }