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High-Throughput SuperSAGE for Digital Gene Expression Analysis of Multiple Samples Using Next Generation Sequencing
http://hdl.handle.net/10091/17555
http://hdl.handle.net/10091/17555f38d8fca-0da6-49ac-ba65-98d0f67d4ed6
| 名前 / ファイル | ライセンス | アクション |
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High-Throughput_SuperSAGE_Digital_Gene_Expressionn.pdf (310.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2014-05-28 | |||||
| タイトル | ||||||
| タイトル | High-Throughput SuperSAGE for Digital Gene Expression Analysis of Multiple Samples Using Next Generation Sequencing | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1371/journal.pone.0012010 | |||||
| 関連名称 | 10.1371/journal.pone.0012010 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Matsumura, Hideo
× Matsumura, Hideo× Yoshida, Kentaro× Luo, Shujun× Kimura, Eiji× Fujibe, Takahiro× Albertyn, Zayed× Barrero, Roberto A× Krueger, Detlev H× Kahl, Guenter× Schroth, Gary P× Terauchi, Ryohei |
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| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Matsumura, Hideo | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.OCSFbmkF.html | |||||
| 出版者 | ||||||
| 出版者 | PUBLIC LIBRARY SCIENCE | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | PLoS One 5(8):e12010 (2010) | |||||
| 書誌情報 |
PLOS ONE 巻 5, 号 8, p. e12010, 発行日 2010-08-12 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High-Throughput (HT-) SuperSAGE''. SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. In the present protocol, index (barcode) sequences are employed to discriminate tags from different samples. Such barcodes allow researchers to analyze digital tags from transcriptomes of many samples in a single sequencing run by simply pooling the libraries. Here, we demonstrated that HT-SuperSAGE provided highly sensitive, reproducible and accurate digital gene expression data. By increasing throughput for analysis in HT-SuperSAGE, various applications are foreseen and several examples are provided in the present study, including analyses of laser-microdissected cells, biological replicates and tag extraction using different anchoring enzymes. | |||||
| 資源タイプ（コンテンツの種類） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1932-6203 | |||||
| PubMed | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=20700453 | |||||
| 関連名称 | 20700453 | |||||
| 権利 | ||||||
| 権利情報 | Copyright© 2010 Matsumura et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| WoS | ||||||
| 表示名 | Web of Science | |||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000280687900010 | |||||
