{"created":"2021-03-01T06:16:18.091721+00:00","id":13375,"links":{},"metadata":{"_buckets":{"deposit":"b56bc7df-79ed-41d8-ad50-e337d013b33d"},"_deposit":{"id":"13375","owners":[],"pid":{"revision_id":0,"type":"depid","value":"13375"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00013375","sets":["1309:1310"]},"author_link":["40624","40625","40626","40627","40628","40629","40630"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"299","bibliographicPageStart":"293","bibliographicVolumeNumber":"113","bibliographic_titles":[{"bibliographic_title":"JOURNAL OF BIOSCIENCE AND BIOENGINEERING"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Chitiniphilus shinanonensis type strain SAY3(T) is a strongly chitinolytic bacterium, originally isolated from the moat water in Ueda, Japan. To elucidate the chitinolytic activity of this strain, 15 genes (chiA-chiO) coding for putative chitin-degrading enzymes were isolated from a genomic library. Sequence analysis revealed the genes comprised 12 family 18 chitinases, a family 19 chitinase, a family 20 beta-N-acetylglucosaminidase, and a polypeptide with a chitin-binding domain but devoid of a catalytic domain. Two operons were detected among the sequences: chiCDEFG and chiLM. The gene coding for the polypeptide (chiN) showed sequence similarity to family 19 chitinases and was successfully expressed in Escherichia colt. ChiN demonstrated a multi-domain structure, composed of the N-terminal, two chitin-binding domains connected by a Pro- and Thr-rich linker, and a family 19 catalytic domain located at the C-terminus. The recombinant protein rChiN catalyzed an endo-type cleavage of N-acetyl-D-glucosamine oligomers, and also degraded insoluble chitin and soluble chitosan (degree of deacetylation of 80%). rChiN exhibited an inhibitory effect on hyphal growth of the fungus Trichoderma reesei. The chitin-binding domains of ChiN likely play an important role in the degradation of insoluble chitin, and are responsible for a growth inhibitory effect on fungi. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"JOURNAL OF BIOSCIENCE AND BIOENGINEERING. 113(3):293-299 (2012)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Nogawa, Masahiro","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.gNcajNnU.html"},{"subitem_link_text":"Taguchi, Goro","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html"},{"subitem_link_text":"Shimosaka, Makoto","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html"}]},"item_6_link_67":{"attribute_name":"WoS","attribute_value_mlt":[{"subitem_link_url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000302505700004"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"SOC BIOSCIENCE BIOENGINEERING JAPAN"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"22178339"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=22178339","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/j.jbiosc.2011.10.018"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.jbiosc.2011.10.018","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright© 2011, The Society for Biotechnology, Japan."}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1389-1723","subitem_source_identifier_type":"ISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11307678","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Huang,  Lanxiang"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Garbulewska,  Ewelina"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato,  Kazuaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato,  Yuichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nogawa,  Masahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taguchi,  Goro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimosaka,  Makoto"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-28"}],"displaytype":"detail","filename":"Isolation_Genes_Coding_Chitin-Degrading_Enzymes.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Isolation_Genes_Coding_Chitin-Degrading_Enzymes.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/13375/files/Isolation_Genes_Coding_Chitin-Degrading_Enzymes.pdf"},"version_id":"596af9c9-e582-43fe-aa37-e96b70cfe697"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Chitiniphilus shinanonensis","subitem_subject_scheme":"Other"},{"subitem_subject":"Family 19 chitinase","subitem_subject_scheme":"Other"},{"subitem_subject":"Chitin-binding domain","subitem_subject_scheme":"Other"},{"subitem_subject":"Fungal growth inhibition","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1310"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-01-18"},"publish_date":"2013-01-18","publish_status":"0","recid":"13375","relation_version_is_last":true,"title":["Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T03:46:34.361064+00:00"}