| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-01-18 |
| タイトル |
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|
タイトル |
Bacillus subtilis Cw1Q (previous YjbJ) is a bifunctional enzyme exhibiting muramidase and soluble-lytic transglycosylase activities |
| 言語 |
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言語 |
eng |
| DOI |
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関連識別子 |
https://doi.org/10.1016/j.bbrc.2010.07.001 |
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関連名称 |
10.1016/j.bbrc.2010.07.001 |
| キーワード |
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主題 |
Cell wall, Peptidoglycan hydrolase, Bifunctional domain, Bacillus subtilis, Site-directed mutagenesis |
| 資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 著者 |
Sudiarta, I. Putu
Fukushima, Tatsuya
Sekiguchi, Junichi
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| 出版者 |
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出版者 |
ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 引用 |
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内容記述 |
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 398(3):606-612 (2010) |
| 書誌情報 |
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
巻 398,
号 3,
p. 606-612,
発行日 2010-07-30
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| 抄録 |
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内容記述 |
CwIQ (previous YjbJ) is one of the putative cell wall hydrolases in Bacillus subtilis. Its domain has an amino acid sequence similar to the soluble-lytic transglycosylase (SLT) of Escherichia coli Slt70 and also goose lysozyme (muramidase). To characterize the enzyme, the domain of CwIQ was cloned and expressed in E. coil. The purified CwIQ protein exhibited cell wall hydrolytic activity. Surprisingly, RP-HPLC, mass spectrometry (MS), and MS/MS analyses showed that CwIQ produces two products, 1,6-anhydro-N-acetylmuramic acid and N-acetylmuramic acid, thus indicating that CwIQ is a bifunctional enzyme. The site-directed mutagenesis revealed that glutamic acid 85 (Glu-85) is an amino acid residue essential to both activities. (C) 2010 Elsevier Inc. All rights reserved. |
| 資源タイプ（コンテンツの種類） |
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0006-291X |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00564395 |
| PubMed |
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識別子タイプ |
PMID |
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関連識別子 |
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=20609359 |
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関連名称 |
20609359 |
| 権利 |
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権利情報 |
Copyright© 2010 Elsevier Inc. |
| 出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| WoS |
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URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000280867400048 |
Bacillus_subtilis_CwlQ_bifunctional_enzyme.pdf (284.2 kB)
