{"created":"2021-03-01T06:16:18.456207+00:00","id":13381,"links":{},"metadata":{"_buckets":{"deposit":"f030b677-26af-482c-b533-246dde8a92cb"},"_deposit":{"id":"13381","owners":[],"pid":{"revision_id":0,"type":"depid","value":"13381"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00013381","sets":["1309:1310"]},"author_link":["40652","40653","40654"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-07-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"612","bibliographicPageStart":"606","bibliographicVolumeNumber":"398","bibliographic_titles":[{"bibliographic_title":"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"CwIQ (previous YjbJ) is one of the putative cell wall hydrolases in Bacillus subtilis. Its domain has an amino acid sequence similar to the soluble-lytic transglycosylase (SLT) of Escherichia coli Slt70 and also goose lysozyme (muramidase). To characterize the enzyme, the domain of CwIQ was cloned and expressed in E. coil. The purified CwIQ protein exhibited cell wall hydrolytic activity. Surprisingly, RP-HPLC, mass spectrometry (MS), and MS/MS analyses showed that CwIQ produces two products, 1,6-anhydro-N-acetylmuramic acid and N-acetylmuramic acid, thus indicating that CwIQ is a bifunctional enzyme. The site-directed mutagenesis revealed that glutamic acid 85 (Glu-85) is an amino acid residue essential to both activities. (C) 2010 Elsevier Inc. All rights reserved.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 398(3):606-612 (2010)","subitem_description_type":"Other"}]},"item_6_link_67":{"attribute_name":"WoS","attribute_value_mlt":[{"subitem_link_text":"Web of Science","subitem_link_url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000280867400048"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ACADEMIC PRESS INC ELSEVIER SCIENCE"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"20609359"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=20609359","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/j.bbrc.2010.07.001"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbrc.2010.07.001","subitem_relation_type_select":"DOI"}}]},"item_6_rights_62":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright© 2010 Elsevier Inc."}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"ISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00564395","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sudiarta,  I. Putu"}],"nameIdentifiers":[{"nameIdentifier":"40652","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fukushima,  Tatsuya"}],"nameIdentifiers":[{"nameIdentifier":"40653","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sekiguchi,  Junichi"}],"nameIdentifiers":[{"nameIdentifier":"40654","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-28"}],"displaytype":"detail","filename":"Bacillus_subtilis_CwlQ_bifunctional_enzyme.pdf","filesize":[{"value":"284.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Bacillus_subtilis_CwlQ_bifunctional_enzyme.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/13381/files/Bacillus_subtilis_CwlQ_bifunctional_enzyme.pdf"},"version_id":"054f2a70-b70c-4f44-b752-e62ff51c7c7a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cell wall","subitem_subject_scheme":"Other"},{"subitem_subject":"Peptidoglycan hydrolase","subitem_subject_scheme":"Other"},{"subitem_subject":"Bifunctional domain","subitem_subject_scheme":"Other"},{"subitem_subject":"Bacillus subtilis","subitem_subject_scheme":"Other"},{"subitem_subject":"Site-directed mutagenesis","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Bacillus subtilis Cw1Q (previous YjbJ) is a bifunctional enzyme exhibiting muramidase and soluble-lytic transglycosylase activities","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Bacillus subtilis Cw1Q (previous YjbJ) is a bifunctional enzyme exhibiting muramidase and soluble-lytic transglycosylase activities","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1310"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-01-18"},"publish_date":"2013-01-18","publish_status":"0","recid":"13381","relation_version_is_last":true,"title":["Bacillus subtilis Cw1Q (previous YjbJ) is a bifunctional enzyme exhibiting muramidase and soluble-lytic transglycosylase activities"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T03:46:41.490962+00:00"}