{"created":"2021-03-01T06:16:20.408223+00:00","id":13413,"links":{},"metadata":{"_buckets":{"deposit":"28a72d55-03a8-4e62-a926-526099870d70"},"_deposit":{"id":"13413","owners":[],"pid":{"revision_id":0,"type":"depid","value":"13413"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00013413","sets":["1309:1310"]},"author_link":["40794","40795","40796","40797","40798","40799","40800"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"1145","bibliographicPageStart":"1139","bibliographicVolumeNumber":"73","bibliographic_titles":[{"bibliographic_title":"THERIOGENOLOGY"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Inhibition of Rho-associated kinase (ROCK) activity promoted recovery and growth of frozen-thawed human embryonic stem cells. The primary objective was to determine if a ROCK inhibitor (Y-27632) in post-thaw culture medium unproved revivability of vitrified IVP bovine blastocysts. Expanding or expanded blastocysts (7 d after IVF) were vitrified (minimum volume cooling procedure, using a Cryotop) in 15% ethylene glycol. 15% DMSO and 0 5 M sucrose When post-warm blastocysts were cultured in inSOF medium, survival rate (re-expansion of blastocoel at 24 h of culture) was improved (P < 0 05) by the addition of 10 mu M Y-27632 (94 9 +/- 2 4%, mean +/- SEM) compared to a control (78 0 +/- 6 0%) Conversely. after 48 h of culture, there were no significant differences in hatching late (62.8 +/- 11 1 vs 59 6 +/- 9.4%) and mean total cell number (135 2 +/- 13 1 vs. 146 7 +/- 13 3) In non-vitrified IVP bovine blastocysts, the hatching rate on Day 9 was improved by Y-27632 (91 7 +/- 3 8 vs 54 7 +/- 8 9%. P < 0 05). with no difference in mean total cell number of blastocysts (230 0 +/- 23 0 vs 191 2 +/- 22 2, P = 0 23). In an additional experiment, Y-27632 was added to culture medium on either Day 0, Day 2. or Day 4 (and remained present until Day 8). resulting in no improvement in blastocyst yield compared to a control group (7.5 +/- 2 1, 31 4 +/- 2 3, 36 2 +/- 3.2. and 28.6 +/- 6.9%. respectively) In conclusion, adding a ROCK inhibitor to post-thaw culture medium improved revivability of IVP bovine blastocysts after vitrification and warming","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"THERIOGENOLOGY. 73(8):1139-1145 (2010)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Hochi, S","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html"}]},"item_6_link_67":{"attribute_name":"WoS","attribute_value_mlt":[{"subitem_link_text":"Web of Science","subitem_link_url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000276801400016"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ELSEVIER SCIENCE INC"}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"20171722"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=20171722","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/j.theriogenology.2010.01.015"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.theriogenology.2010.01.015","subitem_relation_type_select":"DOI"}}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0093-691X","subitem_source_identifier_type":"ISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00862972","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hochi,  S"}],"nameIdentifiers":[{"nameIdentifier":"40794","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Abdalla,  H"}],"nameIdentifiers":[{"nameIdentifier":"40795","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hara,  H"}],"nameIdentifiers":[{"nameIdentifier":"40796","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimoda,  M"}],"nameIdentifiers":[{"nameIdentifier":"40797","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Morita,  H"}],"nameIdentifiers":[{"nameIdentifier":"40798","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kuwayama,  M"}],"nameIdentifiers":[{"nameIdentifier":"40799","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirabayashi,  M"}],"nameIdentifiers":[{"nameIdentifier":"40800","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-28"}],"displaytype":"detail","filename":"THE73_8_1139.pdf","filesize":[{"value":"129.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"THE73_8_1139.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/13413/files/THE73_8_1139.pdf"},"version_id":"7a5e68de-42ec-4627-a9fa-badf0ad90e42"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Bovine blastocysts","subitem_subject_scheme":"Other"},{"subitem_subject":"IVF","subitem_subject_scheme":"Other"},{"subitem_subject":"Rho-associated coiled-coil kinase","subitem_subject_scheme":"Other"},{"subitem_subject":"Vitrification","subitem_subject_scheme":"Other"},{"subitem_subject":"Y-27632","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Stimulatory effect of Rho-associated coiled-coil kinase (ROCK) inhibitor on revivability of in vitro-produced bovine blastocysts after vitrification","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Stimulatory effect of Rho-associated coiled-coil kinase (ROCK) inhibitor on revivability of in vitro-produced bovine blastocysts after vitrification","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1310"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-12-19"},"publish_date":"2012-12-19","publish_status":"0","recid":"13413","relation_version_is_last":true,"title":["Stimulatory effect of Rho-associated coiled-coil kinase (ROCK) inhibitor on revivability of in vitro-produced bovine blastocysts after vitrification"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T04:27:46.670377+00:00"}