{"created":"2021-03-01T06:16:22.970047+00:00","id":13455,"links":{},"metadata":{"_buckets":{"deposit":"4979c81f-a179-4983-bdfa-e540e9d85a61"},"_deposit":{"id":"13455","owners":[],"pid":{"revision_id":0,"type":"depid","value":"13455"},"status":"published"},"_oai":{"id":"oai:soar-ir.repo.nii.ac.jp:00013455","sets":["1309:1310"]},"author_link":["41014","41015","41016","41017","41018","41019","41020"],"item_1628147817048":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"112","bibliographicPageStart":"105","bibliographicVolumeNumber":"157","bibliographic_titles":[{"bibliographic_title":"Plant Science"}]}]},"item_6_description_20":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The enzyme UDP-glucose: hydroxycoumarin 7-O-glucosyltransferase (CGTase), which catalyzes the formation of scopolin from scopoletin, was purified approximately 1200-fold from a culture of 2,4-D-treated tobacco cells (Nicotiana tabacum L. cv. Bright Yellow T-13) with a yield of 7%. Purification to apparent homogeneity, as judged by SDS-PAGE, was achieved by sequential anion-exchange chromatography, hydroxyapatite chromatography, gel filtration, a second round of anion-exchange chromatography, and affinity chromatography on UDP-glucuronic acid agarose. The purified enzyme had a pH optimum of 7.5, an isoelectric point (pI) of 5.0, and a molecular mass of 49 kDa. The enzyme did not require metal cofactors for activity. Its activity was inhibited by Zn2+, Co2+ and Cu2+ ions, as well as by SH-blocking reagents. The K-m values for UDP-glucose, scopoletin and esculetin were 43, 150 and 25 mu M. respectively. A study of the initial rate of the reaction suggested that the reaction proceeded via a sequential mechanism. The purified enzyme preferred hydroxycoumarins as substrates but also exhibited significant activity with flavonoids. A database search using the amino terminus amino acid sequence of CGTase revealed strong homology to the amino acid sequences of other glucosyltransferases in plants.","subitem_description_type":"Abstract"}]},"item_6_description_30":{"attribute_name":"資源タイプ（コンテンツの種類）","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Plant Science. 157(1):105-112 (2000)","subitem_description_type":"Other"}]},"item_6_link_3":{"attribute_name":"信州大学研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Taguchi, G","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html"},{"subitem_link_text":"Hayashida, N","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.HVfmjekV.html"},{"subitem_link_text":"Shimosaka, M","subitem_link_url":"http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html"}]},"item_6_link_67":{"attribute_name":"WoS","attribute_value_mlt":[{"subitem_link_url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000088553500011"}]},"item_6_publisher_4":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_6_relation_46":{"attribute_name":"他の資源との関係：URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.elsevier.com/locate/plantsci"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.elsevier.com/locate/plantsci","subitem_relation_type_select":"URI"}}]},"item_6_relation_47":{"attribute_name":"PubMed","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10940474"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=10940474","subitem_relation_type_select":"PMID"}}]},"item_6_relation_48":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/S0168-9452(00)00270-3"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/S0168-9452(00)00270-3","subitem_relation_type_select":"DOI"}}]},"item_6_source_id_35":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0168-9452","subitem_source_identifier_type":"ISSN"}]},"item_6_source_id_40":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10531279","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Taguchi,  G"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imura,  H"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Maeda,  Y"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kodaira,  R"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hayashida,  N"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimosaka,  M"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okazaki,  M"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-28"}],"displaytype":"detail","filename":"Plant Sci 2000.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Plant Sci 2000.pdf","url":"https://soar-ir.repo.nii.ac.jp/record/13455/files/Plant Sci 2000.pdf"},"version_id":"97b52b55-ab11-4e0c-a5b4-62de41cb230a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Nicotiana tabacum L. cv Bright Yellow","subitem_subject_scheme":"Other"},{"subitem_subject":"glucosyltransferase","subitem_subject_scheme":"Other"},{"subitem_subject":"scopoletin","subitem_subject_scheme":"Other"},{"subitem_subject":"esculetin","subitem_subject_scheme":"Other"},{"subitem_subject":"flavonoid","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Purification and characterization of UDP-glucose: hydroxycoumarin 7-O-glucosyltransferase, with broad substrate specificity from tobacco cultured cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Purification and characterization of UDP-glucose: hydroxycoumarin 7-O-glucosyltransferase, with broad substrate specificity from tobacco cultured cells","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["1310"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-05-26"},"publish_date":"2010-05-26","publish_status":"0","recid":"13455","relation_version_is_last":true,"title":["Purification and characterization of UDP-glucose: hydroxycoumarin 7-O-glucosyltransferase, with broad substrate specificity from tobacco cultured cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-14T03:46:06.038961+00:00"}